*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
VASP (phospho-Ser157) antibody detects endogenous levels of VASP only when phosphorylated at serine 157.
PBS (without Mg2+ and Ca2+) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
The antiserum was produced against synthesized phosphopeptide derived from human VASP around the phosphorylation site of serine 157 (R -V-S(p)-N-A).
Purified by antigen-affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Vasodilator Stimulated Phosphoprotein,Vasp
Cell junction,Cell projection,Cytoplasm,Cytoskeleton,Filopodium membrane,Focal adhesion,Lamellipodium membrane,Tight junction
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]