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VASP (phospho Ser157) antibody

Orthogonal
Anti-VASP (phospho Ser157) antibody used in IHC (Paraffin sections) (IHC-P). GTX66601
Anti-VASP (phospho Ser157) antibody used in Immunoprecipitation (IP). GTX66601
Anti-VASP (phospho Ser157) antibody used in Western Blot (WB). GTX66601
Anti-VASP (phospho Ser157) antibody used in IHC (Paraffin sections) (IHC-P). GTX66601
Anti-VASP (phospho Ser157) antibody used in IHC (Paraffin sections) (IHC-P). GTX66601

Cat. No. GTX66601

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all VASP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic phosphorylated peptide around S157 of human VASP (NP_003361.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vasodilator stimulated phosphoprotein

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton

Background

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-VASP (phospho Ser157) antibody used in IHC (Paraffin sections) (IHC-P). GTX66601

GTX66601 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX66601 VASP (phospho Ser157) antibody.
Dilution : 1:100

Anti-VASP (phospho Ser157) antibody used in Immunoprecipitation (IP). GTX66601

GTX66601 IP Image

IP analysis of A431 cell lysate using GTX66601 VASP (phospho Ser157) antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-VASP (phospho Ser157) antibody used in Western Blot (WB). GTX66601

GTX66601 WB Image

Orthogonal

WB analysis of A431 cells treated by Forskolin (10uM) for 30 minutes after serum-starvation overnight using GTX66601 VASP (phospho Ser157) antibody.
Dilution : 1:2000
Loading : 25μg per lane

Anti-VASP (phospho Ser157) antibody used in IHC (Paraffin sections) (IHC-P). GTX66601

GTX66601 IHC-P Image

IHC-P analysis of human colon tissue using GTX66601 VASP (phospho Ser157) antibody.
Dilution : 1:100

Anti-VASP (phospho Ser157) antibody used in IHC (Paraffin sections) (IHC-P). GTX66601

GTX66601 IHC-P Image

IHC-P analysis of rat brain tissue using GTX66601 VASP (phospho Ser157) antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399