VASP (phospho Ser157) antibody

Cat. No. GTX78924

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse
Package	100 μg ($399)

See all VASP products

Datasheet

View product citations for antibody GTX78924 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:1000
IHC-P	1:50 - 1:100

Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human VASP around the phosphorylation site of serine 157 (R-V-Sp-N-A).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11168068

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

VASP,vasodilatorstimulated phosphoprotein,vasodilator stimulated phosphoprotein

Cellular Localization

Cytoplasm,Cytoskeleton,Cell junction,Focal adhesion,Tight junction,Cell projection,Lamellipodium membrane,Filopodium membrane

Background

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]

Database

Gene ID: 7408 VASP
UniProt: P50552 VASP

Research Area

DATA IMAGES

GTX78924 WB Image

WB analysis of NIH/3T3 cells untreated or treated with forskolin (40μM, 30min) lysates using GTX78924 VASP (phospho Ser157) antibody (Lane 3 and 4) and VASP antibody (Lane 1 and 2).

GTX78924 IHC-P Image

IHC-P analysis of human tonsil carcinoma tissue using GTX78924 VASP (phospho Ser157) antibody.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 399

Qty