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VASP (phospho Ser239) antibody

Anti-VASP (phospho Ser239) antibody used in IHC (Paraffin sections) (IHC-P). GTX38661
Anti-VASP (phospho Ser239) antibody used in Western Blot (WB). GTX38661

Cat. No. GTX38661

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
50 μl ($399)
See all VASP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Product Note

This antibody detects endogenous levels of VASP only when phosphorylated at serine 239.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human VASP around the phosphorylation site of serine 239 (K -V-S(p)-K-Q).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vasodilator stimulated phosphoprotein

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton

Background

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-VASP (phospho Ser239) antibody used in IHC (Paraffin sections) (IHC-P). GTX38661

GTX38661 IHC-P Image

IHC-P analysis of human tonsil carcinoma tissue using GTX38661 VASP (phospho Ser239) antibody.

Anti-VASP (phospho Ser239) antibody used in Western Blot (WB). GTX38661

GTX38661 WB Image

WB analysis of extracts of NIH-3T3 cells untreated or treated with forskolin (40μM, 30min) using VASP antibody (Lane 1 and 2) and GTX38661 VASP (phospho Ser239) antibody(Lane 3 and 4).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399