GeneTex
United States (US)

VASP (phospho Ser239) antibody

GTX38661_20190129_WB_19012901_677.jpg
GTX38661_20190129_IHC-P_19012901_473.jpg

Cat No. GTX38661

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Specificity/Sensitivity

This antibody detects endogenous levels of VASP only when phosphorylated at serine 239.
PROPERTIES

Form

Liquid

Buffer

PBS (without Mg2+ and Ca2+) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human VASP around the phosphorylation site of serine 239 (K -V-S(p)-K-Q).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Vasodilator Stimulated Phosphoprotein,Vasp

Cellular Localization

Cell junction,Cell projection,Cytoplasm,Cytoskeleton,Filopodium membrane,Focal adhesion,Lamellipodium membrane,Tight junction

Background

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX38661_20190129_WB_19012901_677.jpg

GTX38661 WB Image

WB analysis of extracts of NIH-3T3 cells untreated or treated with forskolin (40μM, 30min) using VASP antibody (Lane 1 and 2) and GTX38661 VASP (phospho Ser239) antibody(Lane 3 and 4).

GTX38661_20190129_IHC-P_19012901_473.jpg

GTX38661 IHC-P Image

IHC-P analysis of human tonsil carcinoma tissue using GTX38661 VASP (phospho Ser239) antibody.

Package List Price ($)
$ 219