VAV1 antibody [GT557]

Cat. No. GTX629749

Host	Mouse
Clonality	Monoclonal
Clone Name	GT557
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

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Datasheet
Document

View product citations for antibody GTX629749 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Positive Control

Jurkat , NCI-H929

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VAV1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888149

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vav guanine nucleotide exchange factor 1 , VAV

Background

The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. [provided by RefSeq]

Database

Gene ID: 7409 VAV1
UniProt: P15498 VAV1

Research Area

DATA IMAGES

GTX629749 WB Image

VAV1 antibody [GT557] detects VAV1 protein by Western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg NCI-H929 whole cell lysate/extract
7.5 % SDS-PAGE
VAV1 antibody [GT557] (GTX629749) dilution: 1:1000

GTX629749 ICC/IF Image

VAV1 antibody [GT557] detects VAV1 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VAV1 stained by VAV1 antibody [GT557] (GTX629749) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

GTX629749 IHC-P Image

VAV1 antibody [GT557] detects VAV1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
VAV1 stained by VAV1 antibody [GT557] (GTX629749) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations