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VAV1 antibody

Anti-VAV1 antibody used in Western Blot (WB). GTX103489
Anti-VAV1 antibody used in Western Blot (WB). GTX103489
Anti-VAV1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103489

Cat No. GTX103489

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Positive Control

Jurkat , NCI-H929 , BCL-1

Predict Reactivity

Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VAV1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

vav guanine nucleotide exchange factor 1 , VAV

Background

The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-VAV1 antibody used in Western Blot (WB). GTX103489

GTX103489 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VAV1 antibody (GTX103489) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VAV1 antibody used in Western Blot (WB). GTX103489

GTX103489 WB Image

VAV1 antibody detects VAV1 protein by western blot analysis.
A. 30 µg BCL-1 whole cell lysate/extract
7.5% SDS-PAGE
VAV1 antibody (GTX103489) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VAV1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103489

GTX103489 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human serous ovarian cancer, using VAV1(GTX103489) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

VAV1 antibody Cat No. GTX103489

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 28-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : Jurkat cells
Amount used : 15μg
Blocking : 5% milk, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169