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VAV1 antibody

Orthogonal
Anti-VAV1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103489
Anti-VAV1 antibody used in Western Blot (WB). GTX103489
Anti-VAV1 antibody used in Western Blot (WB). GTX103489
Anti-VAV1 antibody used in Western Blot and Immunoprecipitation (WB IP). GTX103489
Anti-VAV1 antibody used in Western Blot (WB). GTX103489

Cat. No. GTX103489

Reference 3
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all VAV1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Positive Control

BCL-1

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VAV1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952539

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vav guanine nucleotide exchange factor 1 , VAV

Background

The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VAV1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103489

GTX103489 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human serous ovarian cancer, using VAV1(GTX103489) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VAV1 antibody used in Western Blot (WB). GTX103489

GTX103489 WB Image

VAV1 antibody detects VAV1 protein by western blot analysis.
A. 30 μg BCL-1 whole cell lysate/extract
7.5% SDS-PAGE
VAV1 antibody (GTX103489) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VAV1 antibody used in Western Blot (WB). GTX103489

GTX103489 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with VAV1 antibody (GTX103489) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VAV1 antibody used in Western Blot and Immunoprecipitation (WB IP). GTX103489

GTX103489 WB IP Image

Orthogonal

The data was published in the journal PLoS One in 2013.PMID: 23620790

Anti-VAV1 antibody used in Western Blot (WB). GTX103489

GTX103489 WB Image

Orthogonal

The data was published in the journal PLoS One in 2013.PMID: 23620790

REFERENCE

Application Reference

I Nakade et al. Research Square 2023; Bruton’s tyrosine kinase is a possible therapeutic target in myeloperoxidase-antineutrophil cytoplasmic antibody-associated vasculitis
Application : WB
Reactivity : Human
Terasaki M et al. Cancer Genomics Proteomics 2021; 18 (2) : 133-146 A Fucoxanthinol Induces Apoptosis in a Pancreatic Intraepithelial Neoplasia Cell.
Application : WB
Reactivity : Mouse
Chiang YJ et al. PLoS One 2013; 8 (4) : e61761 CBAP functions as a novel component in chemokine-induced ZAP70-mediated T-cell adhesion and migration.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

VAV1 antibody Cat. No. GTX103489

Rating
( Average 3 based on 1 users reviews)
Application
Western Blot(WB)
( Average 3 based on 1 users reviews)
Anti-VAV1 antibody used in Western Blot (WB). GTX103489
Date : Anonymous submitted on 28-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : Jurkat cells
Amount used : 15μg
Blocking : 5% milk, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169