VAV1 (phospho Tyr174) antibody [HL4315]

Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with VAV1 (phospho Tyr174) antibody [HL4315] (GTX643562) diluted at 1:3000 and VAV1 antibody (GTX103489) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VAV1 (phospho Tyr174) antibody [HL4315] (GTX643562) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.