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VAV1 (phospho Tyr174) antibody

Anti-VAV1 (phospho Tyr174) antibody used in Western Blot (WB). GTX132787

Cat No. GTX132787

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Positive Control

Jurkat (serum starvation for 15 min) , Jurkat (serum starvation for 15 min, then 0.13 μg/mL anti-T-cell receptor antibody (clone 305) treatment for 5 min)

Predict Reactivity

Mouse, Rat, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human VAV1 (phospho Tyr174). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

vav guanine nucleotide exchange factor 1,VAV

Background

The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-VAV1 (phospho Tyr174) antibody used in Western Blot (WB). GTX132787

GTX132787 WB Image

Untreated (–) and treated (+) Jurkat whole cell extracts (60 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VAV1 Tyr 174 antibody (GTX132787) diluted at 1:1000.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169