GeneTex
United States (US)

VAV2 antibody

VAV2-antibody_WB_GTX16135-1_18121411_544.jpg

Cat No. GTX16135

Host Sheep
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
APPLICATION

Application Note

IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/5000. Predicted molecular weight: 101 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

101 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.08% Sodium Azide; Constituents: PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

His tagged fusion protein, corresponding to amino acids 578-878 of Human VAV2.

Purification

IgG fraction
This antibody is provided as a 0.2 μm sterile filtered solution.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Vav Guanine Nucleotide Exchange Factor 2,Vav-2,Vav2

Background

VAV2 is a ubiquitously expressed structural homolog of the VAV protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type VAV2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a VAV2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.

Database

Research Area

DATA IMAGES
VAV2-antibody_WB_GTX16135-1_18121411_544.jpg

GTX16135 WB Image

HEK293 cells were transfected with either emply expression vector (pSR ) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 u g lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 l/300 g lysate). DPH or SHD alpha Vav2 antiserum (2.5 l/300 g lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 l/300 g lysate). DPH or SHD anti Vav2 antisera (2.5 l/300 g lysate). Western blot Ab - SHD anti Vav2 antibody 1:10000.

Package List Price ($)
$ 329