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VAV2 antibody

Anti-VAV2 antibody used in Western Blot (WB). GTX16136

Cat. No. GTX16136

Host

 Sheep

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
250 μg ($489)
See all VAV2 products

APPLICATION

Application Note

IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/5000. Predicted molecular weight: 101 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

101 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.08% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

His tagged fusion protein, corresponding to amino acids 287-578 of Human VAV2.

Purification

IgG fraction
This antibody is provided as a 0.2 μm sterile filtered solution.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vav guanine nucleotide exchange factor 2 , VAV-2

Background

VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Database

Research Area

DATA IMAGES

Anti-VAV2 antibody used in Western Blot (WB). GTX16136

GTX16136 WB Image

HEK293 cells were transfected with either emply expression vector (pSR ) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 u g lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 l/300 g lysate). DPH or SHD alpha Vav2 antiserum (2.5 l/300 g lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 l/300 g lysate). DPH or SHD anti Vav2 antisera (2.5 l/300 g lysate). Werstern blot Ab - SHD anti Vav2 antibody 1:10000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 489