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VCAM1 / CD106 antibody [N1N2], N-term

Anti-VCAM1 / CD106 antibody [N1N2], N-term used in Western Blot (WB). GTX110684
Anti-VCAM1 / CD106 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110684
Anti-VCAM1 / CD106 antibody [N1N2], N-term used in Western Blot (WB). GTX110684

Cat No. GTX110684

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

K562 , VCAM1 / CD106-transfected 293T

Predict Reactivity

Rabbit(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human VCAM1 / CD106. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10719755

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

vascular cell adhesion molecule 1 , CD106 , INCAM-100

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VCAM1 / CD106 antibody [N1N2], N-term used in Western Blot (WB). GTX110684

GTX110684 WB Image

Sample (30 μg of whole cell lysate)
A: K562
7.5% SDS PAGE
GTX110684 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VCAM1 / CD106 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110684

GTX110684 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using VCAM1 / CD106 (GTX110684) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VCAM1 / CD106 antibody [N1N2], N-term used in Western Blot (WB). GTX110684

GTX110684 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCAM1 / CD106 antibody [N1N2], N-term (GTX110684) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

VCAM1 / CD106 antibody [N1N2], N-term Cat No. GTX110684

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Untreated MG63 whole cell lysate, Lane 2: treated (CXCL13; 3ug/mL) MG63 whole cell lysate, Lane 3: treated (CXCL13; 10ug/mL) MG63 whole cell lysate
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169