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VCAM1 / CD106 antibody

Anti-VCAM1 / CD106 antibody used in IHC (Paraffin sections) (IHC-P). GTX100562
Anti-VCAM1 / CD106 antibody used in IHC (Paraffin sections) (IHC-P). GTX100562
Anti-VCAM1 / CD106 antibody used in Western Blot (WB). GTX100562

Cat No. GTX100562

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
See all VCAM1 / CD106 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

VCAM1-transfected 293T

Predict Reactivity

Rabbit, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VCAM1 / CD106. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

vascular cell adhesion molecule 1 , CD106 , INCAM-100

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-VCAM1 / CD106 antibody used in IHC (Paraffin sections) (IHC-P). GTX100562

GTX100562 IHC-P Image

VCAM1 / CD106 antibody detects VCAM1 / CD106 protein at cell membrane in human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
VCAM1 / CD106 antibody (GTX100562) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Anti-VCAM1 / CD106 antibody used in IHC (Paraffin sections) (IHC-P). GTX100562

GTX100562 IHC-P Image

VCAM1 / CD106 antibody detects VCAM1 / CD106 protein at cell membrane in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
VCAM1 / CD106 antibody (GTX100562) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Anti-VCAM1 / CD106 antibody used in Western Blot (WB). GTX100562

GTX100562 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCAM1 / CD106 antibody (GTX100562) diluted at 1:200000.
REFERENCE
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169