GeneTex
United States (US)

VCAM1 / CD106 antibody

GTX100562_41773_20150909_IHC-P_19011506_131.jpg
GTX100562_39995_20181221_WB_B_19011519_306.jpg
GTX100562_41983_20150909_IHC-P_19011506_633.jpg

Cat No. GTX100562

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

VCAM1-transfected 293T

Predict Reactivity

Rabbit, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VCAM1 / CD106. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD106 antibody, DKFZp779G2333 antibody, INCAM-100 antibody, MGC99561 antibody, VCAM1 antibody, vascular cell adhesion protein 1 antibody, CD106 antigen antibody, vascular cell adhesion molecule 1 antibody

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100562_41773_20150909_IHC-P_19011506_131.jpg

GTX100562 IHC-P Image

VCAM1 / CD106 antibody detects VCAM1 / CD106 protein at cell membrane in human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
VCAM1 / CD106 antibody (GTX100562) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100562_39995_20181221_WB_B_19011519_306.jpg

GTX100562 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCAM1 / CD106 antibody (GTX100562) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100562_41983_20150909_IHC-P_19011506_633.jpg

GTX100562 IHC-P Image

VCAM1 / CD106 antibody detects VCAM1 / CD106 protein at cell membrane in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
VCAM1 / CD106 antibody (GTX100562) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169