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VCP antibody [JM11-15]

Recombinant antibody
Anti-VCP antibody [JM11-15] used in Western Blot (WB). GTX00999
Anti-VCP antibody [JM11-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00999
Anti-VCP antibody [JM11-15] used in Flow cytometry (FACS). GTX00999
Anti-VCP antibody [JM11-15] used in IHC (Paraffin sections) (IHC-P). GTX00999
Anti-VCP antibody [JM11-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00999
Anti-VCP antibody [JM11-15] used in IHC (Paraffin sections) (IHC-P). GTX00999
Anti-VCP antibody [JM11-15] used in IHC (Paraffin sections) (IHC-P). GTX00999
Anti-VCP antibody [JM11-15] used in Western Blot (WB). GTX00999
Anti-VCP antibody [JM11-15] used in IHC (Paraffin sections) (IHC-P). GTX00999
Anti-VCP antibody [JM11-15] used in IHC (Paraffin sections) (IHC-P). GTX00999
Anti-VCP antibody [JM11-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00999

Cat No. GTX00999

Host

Rabbit

Clonality

Monoclonal

Clone Name

JM11-15

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse, Rat, Crucian carp
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:5000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Observed MW

97 kDa. The observed M.W. is based on the following publication. PMID: 28360103

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

valosin containing protein , ALS14 , CMT2Y , HEL220 , HELS70 , IBMPFD , IBMPFD1 , TERA , VCP , p97 , ATPase p97

Cellular Localization

Cytoplasm,Cytosol,Endoplasmic reticulum,Nucleus

Background

This gene encodes a member of the AAA ATPase family of proteins. The encoded protein plays a role in protein degradation, intracellular membrane fusion, DNA repair and replication, regulation of the cell cycle, and activation of the NF-kappa B pathway. This protein forms a homohexameric complex that interacts with a variety of cofactors and extracts ubiquitinated proteins from lipid membranes or protein complexes. Mutations in this gene cause IBMPFD (inclusion body myopathy with paget disease of bone and frontotemporal dementia), ALS (amyotrophic lateral sclerosis) and Charcot-Marie-Tooth disease in human patients. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-VCP antibody [JM11-15] used in Western Blot (WB). GTX00999

GTX00999 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [JM11-15] (GTX00999) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 28360103

Anti-VCP antibody [JM11-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00999

GTX00999 ICC/IF Image

ICC/IF analysis of PFA-fixed A549 cells using GTX00999 VCP antibody [JM11-15].
Red : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-VCP antibody [JM11-15] used in Flow cytometry (FACS). GTX00999

GTX00999 FACS Image

FACS analysis of HL-60 cells using GTX00999 VCP antibody [JM11-15].
Red : primary antibody
Black : unlabelled control
Dilution : 1:50

Anti-VCP antibody [JM11-15] used in IHC (Paraffin sections) (IHC-P). GTX00999

GTX00999 IHC-P Image

IHC-P analysis of mouse colon tissue section using GTX00999 VCP antibody [JM11-15].

Anti-VCP antibody [JM11-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00999

GTX00999 ICC/IF Image

ICC/IF analysis of PFA-fixed SW480 cells using GTX00999 VCP antibody [JM11-15].
Red : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-VCP antibody [JM11-15] used in IHC (Paraffin sections) (IHC-P). GTX00999

GTX00999 IHC-P Image

IHC-P analysis of human liver tissue section using GTX00999 VCP antibody [JM11-15].

Anti-VCP antibody [JM11-15] used in IHC (Paraffin sections) (IHC-P). GTX00999

GTX00999 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX00999 VCP antibody [JM11-15].

Anti-VCP antibody [JM11-15] used in Western Blot (WB). GTX00999

GTX00999 WB Image

WB analysis of crucian-carp heart tissue lysate using GTX00999 VCP antibody [JM11-15].
Dilution : 1:500

Anti-VCP antibody [JM11-15] used in IHC (Paraffin sections) (IHC-P). GTX00999

GTX00999 IHC-P Image

IHC-P analysis of human colon cancer tissue section using GTX00999 VCP antibody [JM11-15].

Anti-VCP antibody [JM11-15] used in IHC (Paraffin sections) (IHC-P). GTX00999

GTX00999 IHC-P Image

IHC-P analysis of human breast cancer tissue section using GTX00999 VCP antibody [JM11-15].

Anti-VCP antibody [JM11-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00999

GTX00999 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX00999 VCP antibody [JM11-15].
Red : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

REFERENCE

There are currently no references for VCP antibody [JM11-15] (GTX00999). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for VCP antibody [JM11-15] (GTX00999). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf