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VCP antibody [N1N2], N-term

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030
Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030
Anti-VCP antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030
Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

Cat No. GTX113030

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Dog
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain , rat brain

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human VCP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952542

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

valosin containing protein , CDC48 , TERA , p97

Cellular Localization

Cytoplasm , cytosol , Nucleus

Background

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [N1N2], N-term (GTX113030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX113030 diluted at 1:5000

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

GTX113030 IHC-P Image

VCP antibody [N1N2], N-term detects VCP protein at nucleus and cytosol on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
VCP antibody [N1N2], N-term (GTX113030) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

GTX113030 IHC-P Image

VCP antibody [N1N2], N-term detects VCP protein at cytoplasm and nucleus in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
VCP antibody [N1N2], N-term (GTX113030) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VCP antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113030

GTX113030 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using VCP(p97)(GTX113030) antibody at 1:200 dilution.

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

VCP antibody detects VCP protein by Western blot analysis.
A. 50 μg rat brain lysate/extract
7.5 % SDS-PAGE
VCP antibody (GTX113030) dilution: 1:1000

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

GTX113030 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using VCP(p97)(GTX113030) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

GTX113030 IHC-P Image

VCP antibody [N1N2], N-term detects VCP protein at cytoplasm and nucleus in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
VCP antibody [N1N2], N-term (GTX113030) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

VCP antibody [N1N2], N-term Cat No. GTX113030

Rating ( Average 5 based on 7 users reviews)
Application
Western Blot(WB) ( Average 5 based on 7 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal FEBS Open Bio in 2018.PMID: 30186749
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal FEBS Open Bio in 2018.PMID: 30186749
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal FEBS Open Bio in 2018.PMID: 30186749
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal FEBS Open Bio in 2018.PMID: 30186749
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Dog
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28655918
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 26678046
Date : Anonymous submitted on 21-Jan-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Primary Antibody : 1:20000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169