APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:10000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain , rat brain , A549 , H1299 , HCT116
Predict Reactivity
Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.3 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human VCP. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1952542
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
valosin containing protein , CDC48 , TERA , p97
Cellular Localization
Cytoplasm , cytosol , Nucleus
Background
The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX113030 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [N1N2], N-term (GTX113030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113030 IHC-P Image
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using VCP antibody [N1N2], N-term (GTX113030) at 1:300 dilution.
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GTX113030 WB Image
Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [N1N2], N-term (GTX113030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113030 IHC-Wm Image
VCP antibody [N1N2], N-term detects Vcp protein on zebrafish by whole mount immunohistochemical analysis. Sample: 1 day-post-fertilization zebrafish embryo. VCP antibody [N1N2], N-term (GTX113030) dilution: 1:100.
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GTX113030 WB Image
Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX113030 diluted at 1:5000
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GTX113030 IHC-P Image
VCP antibody [N1N2], N-term detects VCP protein at nucleus and cytosol on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. VCP antibody [N1N2], N-term (GTX113030) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113030 IHC-P Image
VCP antibody [N1N2], N-term detects VCP protein at cytoplasm and nucleus in human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. VCP antibody [N1N2], N-term (GTX113030) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113030 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using VCP(p97)(GTX113030) antibody at 1:200 dilution.
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GTX113030 WB Image
VCP antibody detects VCP protein by Western blot analysis. A. 50 μg rat brain lysate/extract 7.5 % SDS-PAGE VCP antibody (GTX113030) dilution: 1:1000
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GTX113030 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using VCP(p97)(GTX113030) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113030 IHC-P Image
VCP antibody [N1N2], N-term detects VCP protein at cytoplasm and nucleus in human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. VCP antibody [N1N2], N-term (GTX113030) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
REVIEW
VCP antibody [N1N2], N-term
Cat. No. GTX113030
Rating
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( Average 5 based on 7 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 7 users reviews)
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Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal FEBS Open Bio in 2018.
PMID: 30186749
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal FEBS Open Bio in 2018.
PMID: 30186749
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal FEBS Open Bio in 2018.
PMID: 30186749
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal FEBS Open Bio in 2018.
PMID: 30186749
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Dog
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2017.
PMID: 28655918
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2015.
PMID: 26678046
Date :
Anonymous submitted on 21-Jan-2020
Application Tested :
WB
Sample Species :
Hu
Sample :
HeLa cells
Primary Antibody :
1:20000, 4°C, 16Hr