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VCP antibody [N1N2], N-term

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in IHC (Whole mount) (IHC-Wm). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030
Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030
Anti-VCP antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030
Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

Cat. No. GTX113030

Reference 21
7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm, IP

Reactivity

 Human, Mouse, Rat, Zebrafish, Dog
Package
100 μl ($399),
25 μl ($169)
See all VCP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

Mouse brain , rat brain , A549 , H1299 , HCT116

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human VCP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952542

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

valosin containing protein , CDC48 , TERA , p97

Cellular Localization

Cytoplasm , cytosol , Nucleus

Background

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [N1N2], N-term (GTX113030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

GTX113030 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using VCP antibody [N1N2], N-term (GTX113030) at 1:300 dilution.

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [N1N2], N-term (GTX113030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VCP antibody [N1N2], N-term used in IHC (Whole mount) (IHC-Wm). GTX113030

GTX113030 IHC-Wm Image

VCP antibody [N1N2], N-term detects Vcp protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
VCP antibody [N1N2], N-term (GTX113030) dilution: 1:100.
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX113030 diluted at 1:5000

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

GTX113030 IHC-P Image

VCP antibody [N1N2], N-term detects VCP protein at nucleus and cytosol on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
VCP antibody [N1N2], N-term (GTX113030) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

GTX113030 IHC-P Image

VCP antibody [N1N2], N-term detects VCP protein at cytoplasm and nucleus in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
VCP antibody [N1N2], N-term (GTX113030) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VCP antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113030

GTX113030 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using VCP(p97)(GTX113030) antibody at 1:200 dilution.

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

VCP antibody detects VCP protein by Western blot analysis.
A. 50 μg rat brain lysate/extract
7.5 % SDS-PAGE
VCP antibody (GTX113030) dilution: 1:1000

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

GTX113030 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using VCP(p97)(GTX113030) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

GTX113030 IHC-P Image

VCP antibody [N1N2], N-term detects VCP protein at cytoplasm and nucleus in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
VCP antibody [N1N2], N-term (GTX113030) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Ryouichi Tsunedomi et al. BMC Cancer 2022; 22 (1) : 260 Elevated expression of RAB3B plays important roles in chemoresistance and metastatic potential of hepatoma cells.
Application : WB
Reactivity : Human
Kitamura N et al. J Biochem 2021; 170 (1) : 131-138 Protein phosphatase 6 promotes neurite outgrowth by promoting mTORC2 activity in N2a cells.
Fujiwara N et al. Biochem Biophys Res Commun 2021; 552 : 191-195 Protein phosphatase 6 dissociates the Beclin 1/Vps34 complex and inhibits autophagy.
Fujiwara Y et al. Pancreas 2021; 50 (3) : 405-413 Pancreatic Cancer Stem-Like Cells With High Calreticulin Expression Associated With Immune Surveillance.
Application : WB
Reactivity : Human
Fujimoto T et al. Oncol Lett 2021; 21 (1) : 30 Cathepsin B is highly expressed in pancreatic cancer stem-like cells and is associated with patients' surgical outcomes.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

VCP antibody [N1N2], N-term Cat. No. GTX113030

Rating
( Average 5 based on 7 users reviews)
Application
Western Blot(WB)
( Average 5 based on 7 users reviews)
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal FEBS Open Bio in 2018.PMID: 30186749
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal FEBS Open Bio in 2018.PMID: 30186749
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal FEBS Open Bio in 2018.PMID: 30186749
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal FEBS Open Bio in 2018.PMID: 30186749
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Dog
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28655918
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 26678046
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Date : Anonymous submitted on 21-Jan-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Primary Antibody : 1:20000, 4°C, 16Hr
Show More +
Submit a Review
Application Reference
Ryouichi Tsunedomi et al. BMC Cancer 2022; 22 (1):260 Elevated expression of RAB3B plays important roles in chemoresistance and metastatic potential of hepatoma cells.
Application : WB
Reactivity : Human
Kitamura N et al. J Biochem 2021; 170 (1):131-138 Protein phosphatase 6 promotes neurite outgrowth by promoting mTORC2 activity in N2a cells.
Fujiwara N et al. Biochem Biophys Res Commun 2021; 552:191-195 Protein phosphatase 6 dissociates the Beclin 1/Vps34 complex and inhibits autophagy.
Fujiwara Y et al. Pancreas 2021; 50 (3):405-413 Pancreatic Cancer Stem-Like Cells With High Calreticulin Expression Associated With Immune Surveillance.
Application : WB
Reactivity : Human
Fujimoto T et al. Oncol Lett 2021; 21 (1):30 Cathepsin B is highly expressed in pancreatic cancer stem-like cells and is associated with patients' surgical outcomes.
Application : WB
Reactivity : Human
Fujiwara N et al. Cancer Sci 2020; 111 (12):4371-4380 Autophagy regulates levels of tumor suppressor enzyme protein phosphatase 6.
Application : WB
Reactivity : Human
Ikeda S et al. J Biochem 2020; Involvement of PP2A Methylation in the Adipogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cell.
Application : WB
Reactivity : Mouse
Tsuji S et al. J Vet Med Sci 2019; Efficacy of an anti-cancer strategy targeting SET in canine osteosarcoma.
Application : WB
Reactivity : Dog
Chernis N et al. Am J Physiol Cell Physiol 2019; Bisphenol AF promotes inflammation in human white adipocytes.
Application : WB
Reactivity : Human
Yabe R et al. FEBS Open Bio 2018; 8 (9):1486-1496 A stable association with PME-1 may be dispensable for PP2A demethylation - implications for the detection of PP2A methylation and immunoprecipitation.
Ryotaro Yabe et al. FEBS openbio 2018; (Epub) A stable association with PME-1 may be dispensable for PP2A demethylation: Implications for the detection of PP2A methylation and immunoprecipitation.
Application : WB
Reactivity : Mouse
Koh EH et al. Diabetes 2018; (Epub) mir-30a remodels subcutaneous adipose tissue inflammation to improve * sensitivity in obesity.
Application : WB
Reactivity : Mouse
Kake S et al. Sci Rep 2017; 7 (1):4279 The role of SET/I2PP2A in canine mammary tumors.
Application : WB
Reactivity : Dog
Kake S et al. Oncol Rep 2017; 37 (2):1100-1106 Death-associated protein kinase 3 controls the tumor progression of A549 cells through ERK MAPK/c-Myc signaling.
Reactivity : Human
Fujiwara N et al. J Biol Chem 2016; 291 (20):10858-66 Regulation of Beclin 1 Protein Phosphorylation and Autophagy by Protein Phosphatase 2A (PP2A) and Death-associated Protein Kinase 3 (DAPK3).
Application : WB
Reactivity : Mouse
Tsuji S et al. J Vet Med Sci 2016; (Epub) Anti-tumor effects of * on canine lymphoma.
Application : WB
Reactivity : Dog
Enjoji S et al. J Vet Med Sci 2015; 77 (11):1451-6 The therapeutic effects of SET/I2PP2A inhibitors on canine melanoma.
Application : WB
Reactivity : Dog
Yabe R et al. PLoS One 2015; 10 (12):e0145226 Protein Phosphatase Methyl-Esterase PME-1 Protects Protein Phosphatase 2A from Ubiquitin/Proteasome Degradation.
Application : WB
Reactivity : Mouse
May EW et al. Pharmacol Res 2014; 90C:43117 identification of up- and down-regulated proteins in *-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of *-associated resistance.
Application : WB
Reactivity : Human
Huang CH et al. J Biol Chem 2014; 289 (7):4444-54 Role of HERP and a HERP-related protein in HRD1-dependent protein degradation at the endoplasmic reticulum.
Application : WB
Reactivity : Human
Huang CH et al. J Biol Chem 2013; 288 (35):25330-9 Derlin2 protein facilitates HRD1-mediated retro-translocation of sonic hedgehog at the endoplasmic reticulum.
Application : WB, IP
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169