VCP antibody [N1N2], N-term

Cat No. GTX113030

Reference 12

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat, Dog

See all VCP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	Assay dependent

Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain , rat brain

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human VCP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Valosin Containing Protein,Cdc48,Tera,P97,Vcp

Cellular Localization

Cytoplasm , cytosol , Nucleus

Background

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]

Database

Gene ID: 7415
UniProt: P55072

Research Area

DATA IMAGES

GTX113030 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX113030 diluted at 1:5000

GTX113030 IHC-P Image

VCP antibody [N1N2], N-term detects VCP protein at nucleus and cytosol on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
VCP antibody [N1N2], N-term (GTX113030) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113030 IHC-P Image

VCP antibody [N1N2], N-term detects VCP protein at cytoplasm and nucleus in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
VCP antibody [N1N2], N-term (GTX113030) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113030 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using VCP(p97)(GTX113030) antibody at 1:200 dilution.

GTX113030 WB Image

VCP antibody detects VCP protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
7.5 % SDS-PAGE
VCP antibody (GTX113030) dilution: 1:1000

GTX113030 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using VCP(p97)(GTX113030) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113030 IHC-P Image

GTX113030 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [N1N2], N-term (GTX113030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE