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VCP antibody [N1N2], N-term

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in IHC (Whole mount) (IHC-Wm). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030
Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

Cat. No. GTX113030

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC-Wm

Reactivity

Human, Mouse, Rat, Zebrafish, Dog
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

Mouse brain , rat brain , A549 , H1299 , HCT116

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human VCP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952542

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

valosin containing protein , CDC48 , TERA , p97

Cellular Localization

Cytoplasm , cytosol , Nucleus

Background

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

GTX113030 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using VCP antibody [N1N2], N-term (GTX113030) at 1:300 dilution.

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [N1N2], N-term (GTX113030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VCP antibody [N1N2], N-term used in IHC (Whole mount) (IHC-Wm). GTX113030

GTX113030 IHC-Wm Image

VCP antibody [N1N2], N-term detects Vcp protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
VCP antibody [N1N2], N-term (GTX113030) dilution: 1:100.

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

VCP antibody detects VCP protein by Western blot analysis.
A. 50 μg rat brain lysate/extract
7.5 % SDS-PAGE
VCP antibody (GTX113030) dilution: 1:1000

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

GTX113030 IHC-P Image

VCP antibody [N1N2], N-term detects VCP protein at cytoplasm and nucleus in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
VCP antibody [N1N2], N-term (GTX113030) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [N1N2], N-term (GTX113030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

GTX113030 IHC-P Image

VCP antibody [N1N2], N-term detects VCP protein at nucleus and cytosol on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
VCP antibody [N1N2], N-term (GTX113030) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

GTX113030 IHC-P Image

VCP antibody [N1N2], N-term detects VCP protein at cytoplasm and nucleus in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
VCP antibody [N1N2], N-term (GTX113030) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX113030 diluted at 1:5000

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

GTX113030 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using VCP(p97)(GTX113030) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28655918

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

The data was published in the journal PLoS One in 2015. PMID: 26678046

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

The data was published in the 2022 in BMC Cancer. PMID: 35277124

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

The data was published in the journal FEBS Open Bio in 2018.PMID: 30186749

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

The data was published in the journal FEBS Open Bio in 2018.PMID: 30186749

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

The data was published in the journal FEBS Open Bio in 2018.PMID: 30186749

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

The data was published in the journal FEBS Open Bio in 2018.PMID: 30186749

REFERENCE

REVIEW

VCP antibody [N1N2], N-term Cat. No. GTX113030

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 21-Jan-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Primary Antibody : 1:20000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169