VCP antibody [N3C2], Internal

Name: VCP antibody [N3C2], Internal
Brand: GeneTex
SKU: GTX113099
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-VCP antibody [N3C2], Internal used in Western Blot (WB). GTX113099

Anti-VCP antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX113099

Cat. No. GTX113099

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all VCP products

View product citations for antibody GTX113099 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:20000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

Rat brain , NIH-3T3 , JC , BCL-1 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VCP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731852

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

valosin containing protein , CDC48 , TERA , p97

Cellular Localization

Cytoplasm , cytosol , Nucleus

Background

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]

Database

Gene ID: 7415 VCP
UniProt: P55072 VCP

Research Area

DATA IMAGES

GTX113099 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX113099 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113099 IHC-P Image

VCP antibody [N3C2], Internal detects VCP protein at nucleus on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
VCP antibody [N3C2], Internal (GTX113099) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113099 WB Image

VCP antibody detects VCP protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody (GTX113099) diluted by 1:1000.

GTX113099 WB Image

VCP antibody [N3C2], Internal detects VCP protein by western blot analysis.
A. 50 μg Rat brain lysate/extract
7.5% SDS-PAGE
VCP antibody [N3C2], Internal (GTX113099) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Christian Marin-Muller et al. Pharmaceutics 2023; 15 : 2038

Application : IHC-P

Submit a Reference

REVIEW