APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:20000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1 , Rat brain
Predict Reactivity
Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human VCP. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10731852
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
valosin containing protein , CDC48 , TERA , p97
Cellular Localization
Cytoplasm , cytosol , Nucleus
Background
The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX113099 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using VCP(GTX113099) antibody at 1:500 dilution.
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GTX113099 IHC-P Image
VCP antibody [N3C2], Internal detects VCP protein at nucleus on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. VCP antibody [N3C2], Internal (GTX113099) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113099 WB Image
VCP antibody detects VCP protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody (GTX113099) diluted by 1:1000.
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GTX113099 WB Image
VCP antibody [N3C2], Internal detects VCP protein by western blot analysis. A. 50 μg Rat brain lysate/extract 7.5% SDS-PAGE VCP antibody [N3C2], Internal (GTX113099) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113099 WB Image
Sample (30 μg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE GTX113099 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for VCP antibody [N3C2], Internal (GTX113099). Be the first to share your publications with this product.
REVIEW
VCP antibody [N3C2], Internal
Cat No. GTX113099
Rating
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( Average 5 based on 1 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 21-Jan-2020
Application Tested :
WB
Sample Species :
Hu
Sample :
HeLa cells
Primary Antibody :
1:20000, 4°C, 16Hr