VCP antibody [N3C2], Internal

Name: VCP antibody [N3C2], Internal
Brand: GeneTex
SKU: GTX113099
Price: 329.00 USD
Rating: 5 (1 reviews)

Anti-VCP antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113099

Anti-VCP antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX113099

Anti-VCP antibody [N3C2], Internal used in Western Blot (WB). GTX113099

Cat No. GTX113099

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($329), 25 μl ($169)

See all VCP products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1 , Rat brain

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VCP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731852

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

valosin containing protein , CDC48 , TERA , p97

Cellular Localization

Cytoplasm , cytosol , Nucleus

Background

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]

Database

Gene ID: 7415 VCP
UniProt: P55072 VCP

Research Area

DATA IMAGES

GTX113099 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using VCP(GTX113099) antibody at 1:500 dilution.

GTX113099 IHC-P Image

VCP antibody [N3C2], Internal detects VCP protein at nucleus on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
VCP antibody [N3C2], Internal (GTX113099) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113099 WB Image

VCP antibody detects VCP protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody (GTX113099) diluted by 1:1000.

GTX113099 WB Image

VCP antibody [N3C2], Internal detects VCP protein by western blot analysis.
A. 50 μg Rat brain lysate/extract
7.5% SDS-PAGE
VCP antibody [N3C2], Internal (GTX113099) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113099 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX113099 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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REVIEW