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VCP antibody

Anti-VCP antibody used in Western Blot (WB). GTX101089
Anti-VCP antibody used in IHC (Frozen sections) (IHC-Fr). GTX101089
Anti-VCP antibody used in Western Blot (WB). GTX101089
Anti-VCP antibody used in Immunoprecipitation (IP). GTX101089
Anti-VCP antibody used in Western Blot (WB). GTX101089
Anti-VCP antibody used in IHC (Paraffin sections) (IHC-P). GTX101089
Anti-VCP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101089

Cat No. GTX101089

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

A431 , H1299 , mouse brain , rat brain , A549 , H1299 , HCT-116

Product Note

Knockdown/Knockout validation was supported by customer review data.

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human VCP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952544

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

valosin containing protein , CDC48 , TERA , p97

Cellular Localization

Cytoplasm , cytosol , Nucleus

Background

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VCP antibody used in Western Blot (WB). GTX101089

GTX101089 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody (GTX101089) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VCP antibody used in IHC (Frozen sections) (IHC-Fr). GTX101089

GTX101089 IHC-Fr Image

VCP antibody detects VCP Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: VCP stained by VCP antibody (GTX101089) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-VCP antibody used in Western Blot (WB). GTX101089

GTX101089 WB Image

VCP antibody detects VCP protein by western blot analysis.
A. 50 μg Rat brain lysate/extract
7.5% SDS-PAGE
VCP antibody (GTX101089) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VCP antibody used in Immunoprecipitation (IP). GTX101089

GTX101089 IP Image

Immunoprecipitation of VCP protein from HeLa whole cell extracts using 5 μg of VCP antibody (GTX101089).
Western blot analysis was performed using VCP antibody (GTX101089) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-VCP antibody used in Western Blot (WB). GTX101089

GTX101089 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX101089 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VCP antibody used in IHC (Paraffin sections) (IHC-P). GTX101089

GTX101089 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using VCP(GTX101089) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VCP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101089

GTX101089 ICC/IF Image

VCP antibody detects VCP protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: VCP protein stained by VCP antibody (GTX101089) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE

REVIEW

VCP antibody Cat No. GTX101089

Rating ( Average 5 based on 4 users reviews)
Application
Western Blot(WB) ( Average 5 based on 4 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Front Neurol in 2019.PMID: 31258504
Date : Anonymous submitted on 20-Jan-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Primary Antibody : 1:20000, 4°C, 16Hr
Date : Anonymous submitted on 03-Jul-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human cardiac fibroblast
Amount used : 30 μg
Blocking : 5% BSA
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : Eey I: ERAD inhibitor eeyarestatin I
Date : Anonymous submitted on 20-May-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 10μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Lane Description : Lane 1: U2OS cells, Lane 2: knockdown of human VCP in U2OS cells
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169