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VCP antibody

Anti-VCP antibody used in Western Blot (WB). GTX101089
Anti-VCP antibody used in Western Blot (WB). GTX101089
Anti-VCP antibody used in IHC (Frozen sections) (IHC-Fr). GTX101089
Anti-VCP antibody used in Western Blot (WB). GTX101089
Anti-VCP antibody used in Immunoprecipitation (IP). GTX101089
Anti-VCP antibody used in Western Blot (WB). GTX101089
Anti-VCP antibody used in IHC (Paraffin sections) (IHC-P). GTX101089
Anti-VCP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101089

Cat No. GTX101089

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

A431 , H1299 , mouse brain , rat brain , A549 , H1299 , HCT-116

Specificity/Sensitivity

Knockdown/Knockout validation was supported by customer review data.

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human VCP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

valosin containing protein,CDC48,TERA,p97

Cellular Localization

Cytoplasm , cytosol , Nucleus

Background

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-VCP antibody used in Western Blot (WB). GTX101089

GTX101089 WB Image

VCP antibody detects VCP protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody (GTX101089) diluted by 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VCP antibody used in Western Blot (WB). GTX101089

GTX101089 WB Image

Sample(30 µg of whole cell lysate)
A:A431(GTX27909)
B:H1299
7.5% SDS PAGE
GTX101089 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VCP antibody used in IHC (Frozen sections) (IHC-Fr). GTX101089

GTX101089 IHC-Fr Image

VCP antibody detects VCP Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: VCP stained by VCP antibody (GTX101089) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-VCP antibody used in Western Blot (WB). GTX101089

GTX101089 WB Image

VCP antibody detects VCP protein by western blot analysis.
A. 50 µg Rat brain lysate/extract
7.5% SDS-PAGE
VCP antibody (GTX101089) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VCP antibody used in Immunoprecipitation (IP). GTX101089

GTX101089 IP Image

Immunoprecipitation of VCP protein from HeLa whole cell extracts using 5 μg of VCP antibody (GTX101089).
Western blot analysis was performed using VCP antibody (GTX101089).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-VCP antibody used in Western Blot (WB). GTX101089

GTX101089 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX101089 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VCP antibody used in IHC (Paraffin sections) (IHC-P). GTX101089

GTX101089 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using VCP(GTX101089) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VCP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101089

GTX101089 ICC/IF Image

VCP antibody detects VCP protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: VCP protein stained by VCP antibody (GTX101089) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE
REVIEW

VCP antibody Cat No. GTX101089

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 03-Jul-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human cardiac fibroblast
Amount used : 30 μg
Blocking : 5% BSA
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : Eey I: ERAD inhibitor eeyarestatin I
Date : Anonymous submitted on 20-May-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 10μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Lane Description : Lane 1: U2OS cells, Lane 2: knockdown of human VCP in U2OS cells
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169