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VCPIP1 antibody [C2C3], C-term

KO/KD
Anti-VCPIP1 antibody [C2C3], C-term used in Western Blot (WB). GTX107169
Anti-VCPIP1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107169
Anti-VCPIP1 antibody [C2C3], C-term used in Western Blot (WB). GTX107169
Anti-VCPIP1 antibody [C2C3], C-term used in Western Blot (WB). GTX107169
Anti-VCPIP1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107169

Cat. No. GTX107169

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all VCPIP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Positive Control

Mouse brain , H1299 , HeLa

Predict Reactivity

Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human VCPIP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952546

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

valosin containing protein interacting protein 1 , DUBA3 , VCIP135

Cellular Localization

Endoplasmic reticulum , Golgi apparatus , Golgi stack

Background

Acts as a deubiquitinating enzyme. Necessary for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP.

Database

Research Area

DATA IMAGES

Anti-VCPIP1 antibody [C2C3], C-term used in Western Blot (WB). GTX107169

GTX107169 WB Image

KO/KD

Wild-type (WT) and VCPIP1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with VCPIP1 antibody [C2C3], C-term (GTX107169) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VCPIP1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107169

GTX107169 ICC/IF Image

VCPIP1 antibody [C2C3], C-term detects VCPIP1 protein at Cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20ºC 100% MeOH for 5 min.
Green: VCPIP1 protein stained by VCPIP1 antibody [C2C3], C-term (GTX107169) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-VCPIP1 antibody [C2C3], C-term used in Western Blot (WB). GTX107169

GTX107169 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5 % SDS PAGE
GTX107169 diluted at 1:5000

Anti-VCPIP1 antibody [C2C3], C-term used in Western Blot (WB). GTX107169

GTX107169 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX107169 diluted at 1:1000

Anti-VCPIP1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107169

GTX107169 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using VCPIP1(GTX107169) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169