GeneTex
United States (US)

VDAC1 antibody [N1C2]

GTX114187_40156_IHC_M_18121410_702.jpg
GTX114187_40156_IHC_18121410_878.jpg
GTX114187_WB_18121410_245.jpg

Cat No. GTX114187

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , *F05 ,*F32 , *F39 , *F90

Predict Reactivity

Rabbit, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VDAC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Voltage Dependent Anion Channel 1,Porin,Vdac-1,Vdac1

Cellular Localization

Mitochondrion outer membrane , Cell membrane

Background

This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.

Database

Research Area

DATA IMAGES
GTX114187_40156_IHC_M_18121410_702.jpg

GTX114187 IHC-P Image

VDAC1 antibody [N1C2] detects VDAC1 protein at mitochondria on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
VDAC1 antibody [N1C2] (GTX114187) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114187_40156_IHC_18121410_878.jpg

GTX114187 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using VDAC1(GTX114187) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114187_WB_18121410_245.jpg

GTX114187 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
VDAC1 antibody
GTX114187 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

VDAC1 antibody [N1C2] Cat No. GTX114187

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 05-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : *F05 cells, *F32 cells, *F39 cells, *F90 cells
Amount used : 30μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169