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VDAC2 antibody [C2C3], C-term

Anti-VDAC2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104745
Anti-VDAC2 antibody [C2C3], C-term used in Western Blot (WB). GTX104745
Anti-VDAC2 antibody [C2C3], C-term used in Western Blot (WB). GTX104745
Anti-VDAC2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104745
Anti-VDAC2 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX104745
Anti-VDAC2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104745

Cat No. GTX104745

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ELISA
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ELISA Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , Mouse liver
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VDAC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

voltage dependent anion channel 2 , POR

Cellular Localization

Mitochondrion outer membrane

Background

This gene encodes a member of the voltage-dependent anion channel pore-forming family of proteins that are considered the main pathway for metabolite diffusion across the mitochondrial outer membrane. The encoded protein is also thought to be involved in the mitochondrial apoptotic pathway via regulation of BCL2-antagonist/killer 1 protein activity. Pseudogenes have been identified on chromosomes 1, 2, 12 and 21, and alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-VDAC2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104745

GTX104745 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using VDAC2(GTX104745) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-VDAC2 antibody [C2C3], C-term used in Western Blot (WB). GTX104745

GTX104745 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with VDAC2 antibody [C2C3], C-term (GTX104745) diluted at 1:1000.

Anti-VDAC2 antibody [C2C3], C-term used in Western Blot (WB). GTX104745

GTX104745 WB Image

Sample (50 µg of whole cell lysate)
A: mouse liver
12% SDS PAGE
GTX104745 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VDAC2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104745

GTX104745 IHC-P Image

VDAC2 antibody [C2C3], C-term detects VDAC2 protein at mitochondria in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
VDAC2 antibody [C2C3], C-term (GTX104745) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VDAC2 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX104745

GTX104745 IP Image

VDAC2 antibody [C2C3], C-term immunoprecipitates VDAC2 protein in IP experiments.
IP samples: HepG2 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of VDAC2 protein by 4 μg VDAC2 antibody [C2C3], C-term (GTX104745)
10 % SDS-PAGE
The immunoprecipitated VDAC2 protein was detected by VDAC2 antibody [C2C3], C-term (GTX104745) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-VDAC2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104745

GTX104745 IHC-P Image

VDAC2 antibody [C2C3], C-term detects VDAC2 protein at mitochondria in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
VDAC2 antibody [C2C3], C-term (GTX104745) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169