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VDAC2 antibody [C2C3], C-term

Anti-VDAC2 antibody [C2C3], C-term used in Western Blot (WB). GTX104745
Anti-VDAC2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104745
Anti-VDAC2 antibody [C2C3], C-term used in Western Blot (WB). GTX104745
Anti-VDAC2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104745
Anti-VDAC2 antibody [C2C3], C-term used in Western Blot (WB). GTX104745
Anti-VDAC2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104745
Anti-VDAC2 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX104745
Anti-VDAC2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104745

Cat. No. GTX104745

Reference 9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all VDAC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ELISA Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Predict Reactivity

Zebrafish, Rabbit, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VDAC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241440

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

voltage dependent anion channel 2 , POR

Cellular Localization

Mitochondrion outer membrane

Background

This gene encodes a member of the voltage-dependent anion channel pore-forming family of proteins that are considered the main pathway for metabolite diffusion across the mitochondrial outer membrane. The encoded protein is also thought to be involved in the mitochondrial apoptotic pathway via regulation of BCL2-antagonist/killer 1 protein activity. Pseudogenes have been identified on chromosomes 1, 2, 12 and 21, and alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VDAC2 antibody [C2C3], C-term used in Western Blot (WB). GTX104745

GTX104745 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with VDAC2 antibody [C2C3], C-term (GTX104745) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VDAC2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104745

GTX104745 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using VDAC2(GTX104745) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-VDAC2 antibody [C2C3], C-term used in Western Blot (WB). GTX104745

GTX104745 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with VDAC2 antibody [C2C3], C-term (GTX104745) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VDAC2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104745

GTX104745 IHC-P Image

VDAC2 antibody [C2C3], C-term detects VDAC2 protein at mitochondria in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
VDAC2 antibody [C2C3], C-term (GTX104745) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VDAC2 antibody [C2C3], C-term used in Western Blot (WB). GTX104745

GTX104745 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with VDAC2 antibody [C2C3], C-term (GTX104745) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VDAC2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104745

GTX104745 IHC-P Image

VDAC2 antibody [C2C3], C-term detects VDAC2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
VDAC2 stained by VDAC2 antibody [C2C3], C-term (GTX104745) diluted at 1:500.
Antigen Retrieval: Citrat

Anti-VDAC2 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX104745

GTX104745 IP Image

VDAC2 antibody [C2C3], C-term immunoprecipitates VDAC2 protein in IP experiments.
IP samples: HepG2 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of VDAC2 protein by 4 μg VDAC2 antibody [C2C3], C-term (GTX104745)
10 % SDS-PAGE
The immunoprecipitated VDAC2 protein was detected by VDAC2 antibody [C2C3], C-term (GTX104745) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-VDAC2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104745

GTX104745 IHC-P Image

VDAC2 antibody [C2C3], C-term detects VDAC2 protein at mitochondria in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
VDAC2 antibody [C2C3], C-term (GTX104745) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Fan S et al. ACS Nano 2024; 18 (4) : 2982-2991 Quantum Sensing of Free Radical Generation in Mitochondria of Single Heart Muscle Cells during Hypoxia and Reoxygenation.
Lin N et al. ACS Cent Sci 2023; 9 (9) : 1784-1798 Quantum Sensing of Free Radicals in Primary Human Granulosa Cells with Nanoscale Resolution.
She H et al. Int J Biol Sci 2023; 19 (10) : 3143-3158 VDAC2 malonylation participates in sepsis-induced myocardial dysfunction via mitochondrial-related ferroptosis.
Sharmin R et al. ACS Sens 2022; 7 (11) : 3326-3334 Intracellular Quantum Sensing of Free-Radical Generation Induced by Acetaminophen (APAP) in the Cytosol, in Mitochondria and the Nucleus of Macrophages.
Application : ICC/IF
Reactivity : Mouse
Chiang-Wen Lee et al. Molecules 2022; 27 (2) Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Fan S et al. ACS Nano 2024; 18 (4):2982-2991 Quantum Sensing of Free Radical Generation in Mitochondria of Single Heart Muscle Cells during Hypoxia and Reoxygenation.
Lin N et al. ACS Cent Sci 2023; 9 (9):1784-1798 Quantum Sensing of Free Radicals in Primary Human Granulosa Cells with Nanoscale Resolution.
She H et al. Int J Biol Sci 2023; 19 (10):3143-3158 VDAC2 malonylation participates in sepsis-induced myocardial dysfunction via mitochondrial-related ferroptosis.
Sharmin R et al. ACS Sens 2022; 7 (11):3326-3334 Intracellular Quantum Sensing of Free-Radical Generation Induced by Acetaminophen (APAP) in the Cytosol, in Mitochondria and the Nucleus of Macrophages.
Application : ICC/IF
Reactivity : Mouse
Chiang-Wen Lee et al. Molecules 2022; 27 (2) Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
Application : WB
Reactivity : Human
Nie L et al. Sci Adv 2021; 7 (21) Quantum monitoring of cellular metabolic activities in single mitochondria.
Chen CY et al. Sci Rep 2021; 11 (1):2377 Cardiac-specific microRNA-125b deficiency induces perinatal death and cardiac hypertrophy.
Application : WB
Reactivity : Mouse
Yi-Wen Lo et al. Journal of Cellular and Molecular Medicine 2015; 19 (4):744-59 mitochondrial proteomics with sirna knockdown to reveal acat1 and mdh2 in the development of *-resistant uterine cancer
Application : WB
Reactivity : Human
Cho CK et al. J Proteome Res 2010; 9 (7):3574-82 Amniotic fluid proteome analysis from Down syndrome pregnancies for biomarker discovery.
Application : ELISA
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169