GeneTex
  • Country / Location Selection

United States (US)

VDAC2 antibody [N1C2]

Anti-VDAC2 antibody [N1C2] used in Western Blot (WB). GTX114876
Anti-VDAC2 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114876
Anti-VDAC2 antibody [N1C2] used in Western Blot (WB). GTX114876

Cat. No. GTX114876

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

HepG2 , HCT116

Predict Reactivity

Zebrafish, Rabbit, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VDAC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177553

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

voltage dependent anion channel 2 , POR

Cellular Localization

Mitochondrion outer membrane

Background

This gene encodes a member of the voltage-dependent anion channel pore-forming family of proteins that are considered the main pathway for metabolite diffusion across the mitochondrial outer membrane. The encoded protein is also thought to be involved in the mitochondrial apoptotic pathway via regulation of BCL2-antagonist/killer 1 protein activity. Pseudogenes have been identified on chromosomes 1, 2, 12 and 21, and alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VDAC2 antibody [N1C2] used in Western Blot (WB). GTX114876

GTX114876 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
B: HCT116
12% SDS PAGE
GTX114876 diluted at 1:1000

Anti-VDAC2 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114876

GTX114876 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using VDAC2(GTX114876) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VDAC2 antibody [N1C2] used in Western Blot (WB). GTX114876

GTX114876 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with VDAC2 antibody [N1C2] (GTX114876) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

VDAC2 antibody [N1C2] Cat. No. GTX114876

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Res in 2018. PMID: 30030520
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169