GeneTex
United States (US)

VDAC2 antibody [N1C2]

GTX114876_40604_WB_18121410_222.jpg
GTX114876_40604_IHC_18121410_378.jpg

Cat No. GTX114876

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Predict Reactivity

Rat, Zebrafish, Rabbit, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VDAC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ23841 antibody, POR antibody, VDAC2 antibody, outer mitochondrial membrane protein porin 2 antibody, voltage-dependent anion-selective channel protein 2 antibody, voltage-dependent anion channel 2 antibody

Cellular Localization

Mitochondrion outer membrane

Background

This gene encodes a member of the voltage-dependent anion channel pore-forming family of proteins that are considered the main pathway for metabolite diffusion across the mitochondrial outer membrane. The encoded protein is also thought to be involved in the mitochondrial apoptotic pathway via regulation of BCL2-antagonist/killer 1 protein activity. Pseudogenes have been identified on chromosomes 1, 2, 12 and 21, and alternative splicing results in multiple transcript variants. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX114876_40604_WB_18121410_222.jpg

GTX114876 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
B: HCT116
12% SDS PAGE
GTX114876 diluted at 1:1000

GTX114876_40604_IHC_18121410_378.jpg

GTX114876 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using VDAC2(GTX114876) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf