APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse liver
Predict Reactivity
Rat, Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human VDP / p115. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10622926
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
USO1 vesicle transport factor , P115 , TAP , VDP
Cellular Localization
Cytoplasm , cytosol , Golgi apparatus membrane; Peripheral membrane protein
Background
The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GM130 and giantin in a sequential manner. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-VDP / p115 antibody [N1N2], N-term used in Western Blot (WB). GTX115114](/templates/default/js/lightbox/images/loading.gif "Anti-VDP / p115 antibody [N1N2], N-term used in Western Blot (WB). GTX115114")
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GTX115114 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with VDP / p115 antibody [N1N2], N-term (GTX115114) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX115114 WB Image
Sample (50 ug of whole cell lysate)
A: Mouse liver
5% SDS PAGE
GTX115114 diluted at 1:1000
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GTX115114 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using VDP/p115(GTX115114) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX115114 ICC/IF Image
VDP / p115 antibody [N1N2], N-term detects VDP / p115 protein at cytoplasm and golgi apparatus by immunofluorescent analysis.Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.Green: VDP / p115 stained by VDP / p115 antibody [N1N2], N-term (GTX115114) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
There are currently no references for VDP / p115 antibody [N1N2], N-term (GTX115114). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for VDP / p115 antibody [N1N2], N-term (GTX115114). Be the first to share your experience with this product.
![Anti-VDP / p115 antibody [N1N2], N-term used in Western Blot (WB). GTX115114](/upload/website/prouct_img/small/GTX115114/GTX115114_43978_20200619_WB_w_23060519_829.webp "Anti-VDP / p115 antibody [N1N2], N-term used in Western Blot (WB). GTX115114")
![Anti-VDP / p115 antibody [N1N2], N-term used in Western Blot (WB). GTX115114](/upload/website/prouct_img/small/GTX115114/GTX115114_40261_WB_M_Liver_w_23060519_844.webp "Anti-VDP / p115 antibody [N1N2], N-term used in Western Blot (WB). GTX115114")
![Anti-VDP / p115 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX115114](/upload/website/prouct_img/small/GTX115114/GTX115114_40261_IHC_w_23060519_365.webp "Anti-VDP / p115 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX115114")
![Anti-VDP / p115 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115114](/upload/website/prouct_img/small/GTX115114/GTX115114_43978_20211224_ICC_IF_w_23060519_829.webp "Anti-VDP / p115 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115114")
![Anti-VDP / p115 antibody [N1N2], N-term used in Western Blot (WB). GTX115114](/upload/website/prouct_img/normal/GTX115114/GTX115114_43978_20200619_WB_w_23060519_829.webp "Anti-VDP / p115 antibody [N1N2], N-term used in Western Blot (WB). GTX115114")
![Anti-VDP / p115 antibody [N1N2], N-term used in Western Blot (WB). GTX115114](/upload/website/prouct_img/normal/GTX115114/GTX115114_40261_WB_M_Liver_w_23060519_844.webp "Anti-VDP / p115 antibody [N1N2], N-term used in Western Blot (WB). GTX115114")
![Anti-VDP / p115 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX115114](/upload/website/prouct_img/normal/GTX115114/GTX115114_40261_IHC_w_23060519_365.webp "Anti-VDP / p115 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX115114")
![Anti-VDP / p115 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115114](/upload/website/prouct_img/normal/GTX115114/GTX115114_43978_20211224_ICC_IF_w_23060519_829.webp "Anti-VDP / p115 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115114")