*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , HeLa , HepG2 , NIH-3T3 , mouse liver
Rat, Bovine, Chicken(>80% identity)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the N-terminus region of human VDP / p115. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Uso1 Vesicle Transport Factor,P115,Tap,Vdp,Uso1
Cytoplasm , cytosol , Golgi apparatus membrane; Peripheral membrane protein
The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GM130 and giantin in a sequential manner. [provided by RefSeq]
GTX115114 WB Image
VDP/p115 antibody [N1N2], N-term detects VDP/p115 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg A431 whole cell extract
C. 30 μg HeLa whole cell extract
D. 30 μg HepG2 whole cell extract
E. 30 μg NIH-3T3 whole cell extract
7.5 % SDS-PAGE
VDP/p115 antibody [N1N2], N-term (GTX115114) dilution: 1:1000
GTX115114 WB Image
Sample (50 ug of whole cell lysate)
A: Mouse liver
5% SDS PAGE
GTX115114 diluted at 1:1000
GTX115114 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using VDP/p115(GTX115114) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min