GeneTex
United States (US)

VDP / p115 antibody [N1N2], N-term

GTX115114_40261_WB_Multiple-species_18121410_761.jpg
GTX115114_40261_WB_M_Liver_18121410_119.jpg
GTX115114_40261_IHC_18121410_137.jpg

Cat No. GTX115114

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3 , mouse liver

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human VDP / p115. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Uso1 Vesicle Transport Factor,P115,Tap,Vdp,Uso1

Cellular Localization

Cytoplasm , cytosol , Golgi apparatus membrane; Peripheral membrane protein

Background

The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GM130 and giantin in a sequential manner. [provided by RefSeq]

Database

DATA IMAGES
GTX115114_40261_WB_Multiple-species_18121410_761.jpg

GTX115114 WB Image

VDP/p115 antibody [N1N2], N-term detects VDP/p115 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg A431 whole cell extract
C. 30 μg HeLa whole cell extract
D. 30 μg HepG2 whole cell extract
E. 30 μg NIH-3T3 whole cell extract
7.5 % SDS-PAGE
VDP/p115 antibody [N1N2], N-term (GTX115114) dilution: 1:1000

GTX115114_40261_WB_M_Liver_18121410_119.jpg

GTX115114 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse liver
5% SDS PAGE
GTX115114 diluted at 1:1000

GTX115114_40261_IHC_18121410_137.jpg

GTX115114 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using VDP/p115(GTX115114) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169