GeneTex
United States (US)

VDP / p115 antibody

GTX115115_41115_WB_M_18121410_482.jpg
GTX115115_41115_WB_18121410_131.jpg
GTX115115_41115_IFA_18121410_471.jpg

Cat No. GTX115115

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro2A , CD830 , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human VDP / p115. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Uso1 Vesicle Transport Factor,P115,Tap,Vdp,Uso1

Cellular Localization

Cytoplasm , cytosol , Golgi apparatus membrane; Peripheral membrane protein

Background

The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GM130 and giantin in a sequential manner. [provided by RefSeq]

Database

DATA IMAGES
GTX115115_41115_WB_M_18121410_482.jpg

GTX115115 WB Image

VDP/p115 antibody detects VDP/p115 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with VDP/p115 antibody (GTX115115) diluted by 1:1000.

GTX115115_41115_WB_18121410_131.jpg

GTX115115 WB Image

VDP/p115 antibody detects VDP/p115 protein by Western blot analysis.
A. 30 µg 293T
B. 30 µg A431
C. 30 µg HeLa
D. 30 µg HepG2
7.5 % SDS-PAGE
VDP/p115 antibody (GTX115115) dilution: 1:1000

GTX115115_41115_IFA_18121410_471.jpg

GTX115115 ICC/IF Image

USO1 antibody detects USO1 protein at Golgi apparatus by immunofluorescent analysis.
Sample: A431 cells were fixed in 100% MeOH for 5 min.
Green: USO1 protein stained by USO1 antibody (GTX115115) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169