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VE-Cadherin antibody [3D5C7]

Anti-VE-Cadherin antibody [3D5C7] used in Western Blot (WB). GTX60676
Anti-VE-Cadherin antibody [3D5C7] used in ELISA (ELISA). GTX60676
Anti-VE-Cadherin antibody [3D5C7] used in Flow cytometry (FACS). GTX60676
Anti-VE-Cadherin antibody [3D5C7] used in Western Blot (WB). GTX60676

Cat. No. GTX60676

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3D5C7

Isotype

 IgG1

Application

 WB, FACS, ELISA

Reactivity

 Human, Rat
Package
100 μg ($409)
See all VE-Cadherin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human VE-Cadherin (AA: 29-223 ) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cadherin 5 , 7B4 , CD144

Cellular Localization

Cell junction,Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-VE-Cadherin antibody [3D5C7] used in Western Blot (WB). GTX60676

GTX60676 WB Image

WB analysis of MCF-7 (1), A549 (2), HUVE-12 (3) cell lysate, and rat lung (4) tissue lysate using GTX60676 VE-Cadherin antibody [3D5C7].

Anti-VE-Cadherin antibody [3D5C7] used in ELISA (ELISA). GTX60676

GTX60676 ELISA Image

ELISA analysis of antigen using GTX60676 VE-Cadherin antibody [3D5C7].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-VE-Cadherin antibody [3D5C7] used in Flow cytometry (FACS). GTX60676

GTX60676 FACS Image

FACS analysis of Jurkat cells using GTX60676 VE-Cadherin antibody [3D5C7].
Green : VE-Cadherin
Purple : negative control

Anti-VE-Cadherin antibody [3D5C7] used in Western Blot (WB). GTX60676

GTX60676 WB Image

WB analysis of human CDH5 recombinant protein using GTX60676 VE-Cadherin antibody [3D5C7].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409