APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
|
Note :
WB
A reduced sample treatment and 7.5% SDS-Page was used.
ICC/IF
Cells on coverslips were fixed with 3% paraformaldehyde and permeabilized with 0.5% Triton X-100 before incubation with antibody BV9.
IHC-Fr
Acetone fixed sections were blocked with horse serum and incubated with antibody BV9 for 30 minutes.
ELISA
Antibody BV9 can function as coat and detector.
Neutralizing/Inhibition
Functions as an antagonist. The antibody was functionally tested by adding 10-50 μg/ml antibody BV9 to cell culture. It blocks VE-cadherin causing a redistribution of VE-cadherin away from intracellular junctions.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody stains the extracellular domain of VE-cadherin. The monoclonal antibody BV9 binds to the extracellular domain (EC3-EC4) of human VE-cadherin (vascular endothelial cadherin).
PROPERTIES
Form
Liquid
Buffer
Filter-sterilized PBS, 0.1% BSA
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
100 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Purification
Protein G purified
Endotoxin
< 24 EU/mg (Determined by LAL assay)
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD144,CDH5,cadherin 5,VE Cadherin,VE-Cadherin,Cadherin 5,7B4
Cellular Localization
Cell junction,Cell membrane
Background
This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. [provided by RefSeq, Nov 2015]
Database
Research Area
REFERENCE
There are currently no references for VE-Cadherin antibody [BV9] (GTX54426). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for VE-Cadherin antibody [BV9] (GTX54426). Be the first to share your experience with this product.
