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VE-Cadherin antibody [GT1369]

Anti-VE-Cadherin antibody [GT1369] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633705
Anti-VE-Cadherin antibody [GT1369] used in Western Blot (WB). GTX633705
Anti-VE-Cadherin antibody [GT1369] used in IHC (Paraffin sections) (IHC-P). GTX633705

Cat. No. GTX633705

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1369

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all VE-Cadherin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human VE-Cadherin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888370

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cadherin 5 , 7B4 , CD144

Cellular Localization

Cell junction , Cell membrane; Single-pass type I membrane protein , Cell junction , Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VE-Cadherin antibody [GT1369] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633705

GTX633705 ICC/IF Image

VE-cadherin antibody [GT1369] detects VE-cadherin protein at cell membrane by immunofluorescent analysis.
Sample: HUVEC cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VE-cadherin protein stained by VE-cadherin antibody [GT1369] (GTX633705) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-VE-Cadherin antibody [GT1369] used in Western Blot (WB). GTX633705

GTX633705 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VE-Cadherin antibody [GT1369] (GTX633705) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-VE-Cadherin antibody [GT1369] used in IHC (Paraffin sections) (IHC-P). GTX633705

GTX633705 IHC-P Image

VE-Cadherin antibody [GT1369] detects VE-Cadherin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
VE-Cadherin stained by VE-Cadherin antibody [GT1369] (GTX633705) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Liu IF et al. Biol Direct 2023; 18 (1) : 9 Long-term administration of Western diet induced metabolic syndrome in mice and causes cardiac microvascular dysfunction, cardiomyocyte mitochondrial damage, and cardiac remodeling involving caveolae and caveolin-1 expression.
Martinez-Sanchez J et al. Bone Marrow Transplant 2020; The induction strategies administered in the treatment of multiple myeloma exhibit a deleterious effect on the endothelium.
Application : ICC/IF
Reactivity : Human
Sheen YS et al. J Pathol 2020; 250 (4) : 452-463 Purpuric drug eruptions induced by EGFR tyrosine kinase inhibitors are associated with IQGAP1-mediated increase in vascular permeability.
Application : ICC/IF
Reactivity : Human
Kachamakova-Trojanowska N et al. Cells 2019; 8 (11) Human iPSCs-Derived Endothelial Cells with Mutation in HNF1A as a Model of Maturity-Onset Diabetes of the Young.
Application : ICC/IF
Reactivity : Human
Kudo H et al. Int Endod J 2018; (Epub) A potential role for the silent information regulator 2 homologue 1 (SIRT1) in periapical periodontitis.
Application : ICC/IF
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169