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VE-Cadherin antibody [GT1369]

Anti-VE-Cadherin antibody [GT1369] used in Western Blot (WB). GTX633705
Anti-VE-Cadherin antibody [GT1369] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633705

Cat No. GTX633705

Host Mouse
Clonality Monoclonal
Clone Name GT1369
Isotype IgG1
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

HUVEC
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human VE-Cadherin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cadherin 5,7B4,CD144

Cellular Localization

Cell junction , Cell membrane; Single-pass type I membrane protein , Cell junction , Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-VE-Cadherin antibody [GT1369] used in Western Blot (WB). GTX633705

GTX633705 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VE-cadherin antibody [GT1369] (GTX633705) diluted at 1:1000.

Anti-VE-Cadherin antibody [GT1369] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633705

GTX633705 ICC/IF Image

VE-cadherin antibody [GT1369] detects VE-cadherin protein at cell membrane by immunofluorescent analysis.
Sample: HUVEC cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VE-cadherin protein stained by VE-cadherin antibody [GT1369] (GTX633705) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE
REVIEW
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SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169