IHC: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
This antibody reacts with a 180 kD protein, known as Flt-1/VEGFR 1 (Vascular Endothelial Growth Factor Receptor 1). The sequence that this antibody recognises is not present in soluble VEGF Receptor 1.
PBS with 0.1% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Synthetic peptide: C-AELERKIACCSPPPDYN, corresponding to amino acids 1312-1328 of Human VEGF Receptor 1.
Protein A purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Fms Related Tyrosine Kinase 1,Flt,Flt-1,Vegfr-1,Vegfr1,Flt1
Type I membrane protein (Flt1) and soluble (SFlt1).
VEGFR 1 is a cell membrane receptor kinase. It has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis.