GeneTex
United States (US)

VEGF Receptor 1 antibody

GTX30653_567_IHC-P_18121410_363.jpg
GTX30653_223_ICC-IF_18121410_415.jpg
GTX30653_568_IHC-P_18121410_756.jpg

Cat No. GTX30653

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100 - 1:2000
ICC/IF 1:500
IHC-P 1:250 - 1:500
Not tested in other applications.

Calculated MW

150 kDa. ( Note )

Specificity/Sensitivity

This antibody targets VEGFR-1 but has significant cross-reactivity with the VEGFR-2 protein.
PROPERTIES

Form

Liquid

Buffer

Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

A synthetic peptide made to an internal region of the mouse VEGF Receptor 1 protein (between residues 800-900) [UniProt# P35969]. There is 87% identity between the immunogen used for this production and the VEGF Receptor 2 protein.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AI323757 antibody , Flt-1 antibody , VEGFR-1 antibody , VEGFR1 antibody , sFlt1 antibody

Cellular Localization

Membrane, Single-pass type I membrane protein

Background

Receptor for VEGF and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.

Research Area

DATA IMAGES
GTX30653_567_IHC-P_18121410_363.jpg

GTX30653 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX30653 VEGF Receptor 1 antibody.
Dilution : 1:500
Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)

GTX30653_223_ICC-IF_18121410_415.jpg

GTX30653 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30653 VEGF Receptor 1 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI

GTX30653_568_IHC-P_18121410_756.jpg

GTX30653 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX30653 VEGF Receptor 1 antibody.
Dilution : 1:500
Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)