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VEGF Receptor 2 antibody [6B11]

Orthogonal
Anti-VEGF Receptor 2 antibody [6B11] used in Activation/Stimulation/Induction (Activation). GTX53462

Cat. No. GTX53462

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6B11

Isotype

 IgG1

Application

 WB, Activation, Neutralizing/Inhibition

Reactivity

 Human
Package
100 μg ($689)
See all VEGF Receptor 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Activation Assay dependent
Neutralizing/Inhibition Assay dependent

Note :


Attenuation of VEGF-induced VEGFR2 tyrosine phosphorylation
Not tested in other applications.

Calculated MW

152 kDa. ( Note )

Product Note

This antibody recognizes hVEGFR-2 in western blot and it shows no cross-reactivity with hVEGFR-1.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

hVEGFR2 N-terminal fragment (N30-200)

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2887818

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD309 , FLK1 , KDR , VEGFR , VEGFR2 , kinase insert domain receptor , VEGF Receptor 2

Cellular Localization

Cell membrane,Cytoplasm,Nucleus,Cytoplasmic vesicle,Early endosome,Cell junction,Secreted

Background

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]

Database

Research Area

DATA IMAGES

Anti-VEGF Receptor 2 antibody [6B11] used in Activation/Stimulation/Induction (Activation). GTX53462

GTX53462 Activation Image

Orthogonal

Activation assay analysis of HUVECs pre-treated with 5.0 and 25 μg/ml GTX53462 VEGF Receptor 2 antibody [6B11] and then stimulated with rhVEGF (10ng/ml) for 30min. The phospho-VEGFR2 was detected by detected with IP-Western for P-Tyrosine.

REFERENCE

Application Reference

Qian YY et al. Int J Ophthalmol 2019; 12 (9) : 1386-1394 Blockade of insulin receptor substrate-1 inhibits biological behavior of choroidal endothelial cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 689