APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
|
Note :
Activation
This antibody can induce hVEGFR2 phosphorylation in HUVECs at > 5.0 μg/mL.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody recognizes hVEGFR-2 in western blot and immunoprecipitation assays. It shows no cross-reactivity with hVEGFR-1.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
hVEGFR2 N-terminal fragment (N30-200)
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD309 , FLK1 , KDR , VEGFR , VEGFR2 , kinase insert domain receptor , VEGF Receptor 2
Cellular Localization
Cell membrane,Cytoplasm,Nucleus,Cytoplasmic vesicle,Early endosome,Cell junction,Secreted
Background
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]
Database
Research Area
DATA IMAGES
![Anti-VEGF Receptor 2 antibody [8A01] used in Activation/Stimulation/Induction (Activation). GTX53464](/templates/default/js/lightbox/images/loading.gif "Anti-VEGF Receptor 2 antibody [8A01] used in Activation/Stimulation/Induction (Activation). GTX53464")
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GTX53464 Activation Image
Activation assay analysis of HUVECs stimulated with 20ng/ml VEGF or treated with 5.0 and 25 μg/ml GTX53464 VEGF Receptor 2 antibody [8A01] (30mins). Phospho-VEGFR2 was detected with IP-Western for P-Tyrosine.
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REFERENCE
There are currently no references for VEGF Receptor 2 antibody [8A01] (GTX53464). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for VEGF Receptor 2 antibody [8A01] (GTX53464). Be the first to share your experience with this product.
![Anti-VEGF Receptor 2 antibody [8A01] used in Activation/Stimulation/Induction (Activation). GTX53464](/upload/website/prouct_img/small/GTX53464/GTX53464_20191119_Activation_w_23060900_578.webp "Anti-VEGF Receptor 2 antibody [8A01] used in Activation/Stimulation/Induction (Activation). GTX53464")
![Anti-VEGF Receptor 2 antibody [8A01] used in Activation/Stimulation/Induction (Activation). GTX53464](/upload/website/prouct_img/normal/GTX53464/GTX53464_20191119_Activation_w_23060900_578.webp "Anti-VEGF Receptor 2 antibody [8A01] used in Activation/Stimulation/Induction (Activation). GTX53464")