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VEGF Receptor 2 antibody

Anti-VEGF Receptor 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50432
Anti-VEGF Receptor 2 antibody used in Western Blot (WB). GTX50432
Anti-VEGF Receptor 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX50432

Cat No. GTX50432

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

152 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.949~953 (K-D-Y-V-G) derived from human VEGF Receptor 2.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

kinase insert domain receptor , CD309 , FLK1 , VEGFR , VEGFR2

Cellular Localization

Cell junction,Endoplasmic reticulum,Cytoplasm,Nucleus,Cytoplasmic vesicle

Background

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]

Database

Research Area

DATA IMAGES

Anti-VEGF Receptor 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50432

GTX50432 ICC/IF Image

ICC/IF analysis of methanol-fixed MCF cells using GTX50432 VEGF Receptor 2 antibody.

Anti-VEGF Receptor 2 antibody used in Western Blot (WB). GTX50432

GTX50432 WB Image

WB analysis of extracts from SKOV3 cells using GTX50432 VEGF Receptor 2 antibody with or without blocking peptide.

Anti-VEGF Receptor 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX50432

GTX50432 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50432 VEGF Receptor 2 antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf