GeneTex
United States (US)

VEGF Receptor 2 (phospho Tyr1054/Tyr1059) antibody

Orthogonal
GTX25473_1781_WB_19011519_818.jpg
GTX25473_659_ICC-IF_19011519_484.jpg
GTX25473_1780_WB_19011519_135.jpg

Cat No. GTX25473

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, FACS
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:250
FACS 1:20
Not tested in other applications.

Calculated MW

152 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1mg/ml BSA, 50% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human VEGFR2 that contains tyrosines 1054 and 1059. The sequence is conserved among multiple species including mouse and rat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD309,FLK1,VEGFR,VEGFR2

Cellular Localization

Cell junction,Cell membrane,Cytoplasm,Cytoplasmic vesicle,Early endosome,Endoplasmic reticulum,Nucleus,Secreted

Background

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]

Database

Research Area

DATA IMAGES
GTX25473_1781_WB_19011519_818.jpg

GTX25473 WB Image

Orthogonal

WB analysis of whole cell extracts (30 µg lysate) of HUVEC (Lane 1), treated with 25ng/mL VEGF for 5 minutes (lane 2), MDA-MB-231 (lane 3), and MCF7 (Lane 4) using GTX25473 VEGF Receptor 2 (phospho Tyr1054/Tyr1059) antibody.
Dilution : 1:1000

GTX25473_659_ICC-IF_19011519_484.jpg

GTX25473 ICC/IF Image

Orthogonal

ICC/IF analysis of HUVEC cell treated with 100ng of VEGF for 30 minutes using GTX25473 VEGF Receptor 2 (phospho Tyr1054/Tyr1059) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes

GTX25473_1780_WB_19011519_135.jpg

GTX25473 WB Image

Orthogonal

WB analysis of PAE cells transfected with a chimeric receptor unstimulated (Lane 1, 2) or stimulated with 40ng/mL CSF-1 for 10 minutes (Lane 3-8) using GTX25473 VEGF Receptor 2 (phospho Tyr1054/Tyr1059) antibody.