*Optimal dilutions/concentrations should be determined by the researcher.
|1:500 - 1:1000
|1:100 - 1:500
|1:100 - 1:200
Not tested in other applications.
0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of VEGF Receptor 2. The exact sequence is proprietary.
Purified by antigen-affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Kinase Insert Domain Receptor,Cd309,Flk1,Vegfr,Vegfr2,Kdr
Cell junction,Cell membrane,Cytoplasm,Cytoplasmic vesicle,Early endosome,Endoplasmic reticulum,Nucleus,Secreted
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]
GTX32403 WB Image
WB analysis of EGF-treated HEK293T (A), EGF-treated NIH3T3 (B) whole cell lysates using GTX32403 VEGF Receptor 2 (phospho Tyr1175) antibody.
GTX32403 ICC/IF Image
ICC/IF analysis of formalin-fixed MCF7 cells using GTX32403 VEGF Receptor 2 (phospho Tyr1175) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
GTX32403 IHC-P Image
IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32403 VEGF Receptor 2 (phospho Tyr1175) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)