GeneTex
United States (US)

VEGF Receptor 2 (phospho Tyr1175) antibody

GTX32403_20190131_WB_19013101_189.jpg
GTX32403_20190131_ICCIF_19013101_739.jpg
GTX32403_20190131_IHC-P_19013101_848.jpg

Cat No. GTX32403

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

152 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of VEGF Receptor 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Kinase Insert Domain Receptor,Cd309,Flk1,Vegfr,Vegfr2,Kdr

Cellular Localization

Cell junction,Cell membrane,Cytoplasm,Cytoplasmic vesicle,Early endosome,Endoplasmic reticulum,Nucleus,Secreted

Background

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]

Database

Research Area

DATA IMAGES
GTX32403_20190131_WB_19013101_189.jpg

GTX32403 WB Image

WB analysis of EGF-treated HEK293T (A), EGF-treated NIH3T3 (B) whole cell lysates using GTX32403 VEGF Receptor 2 (phospho Tyr1175) antibody.

GTX32403_20190131_ICCIF_19013101_739.jpg

GTX32403 ICC/IF Image

ICC/IF analysis of formalin-fixed MCF7 cells using GTX32403 VEGF Receptor 2 (phospho Tyr1175) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX32403_20190131_IHC-P_19013101_848.jpg

GTX32403 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32403 VEGF Receptor 2 (phospho Tyr1175) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)