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VEGF Receptor 2 (phospho Tyr1214) antibody

Anti-VEGF Receptor 2 (phospho Tyr1214) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50152
Anti-VEGF Receptor 2 (phospho Tyr1214) antibody used in Western Blot (WB). GTX50152
Anti-VEGF Receptor 2 (phospho Tyr1214) antibody used in IHC (Paraffin sections) (IHC-P). GTX50152

Cat No. GTX50152

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

152 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 1214 (F-H-Y(p)-D-N) derived from human VEGF Receptor 2.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

kinase insert domain receptor , CD309 , FLK1 , VEGFR , VEGFR2

Cellular Localization

Cell junction,Endoplasmic reticulum,Cytoplasm,Nucleus,Cytoplasmic vesicle

Background

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]

Database

Research Area

DATA IMAGES

Anti-VEGF Receptor 2 (phospho Tyr1214) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50152

GTX50152 ICC/IF Image

ICC/IF analysis of methanol-fixed MCF cells using GTX50152 VEGF Receptor 2 (phospho Tyr1214) antibody.

Anti-VEGF Receptor 2 (phospho Tyr1214) antibody used in Western Blot (WB). GTX50152

GTX50152 WB Image

WB analysis of extracts from SKOV3 cells using GTX50152 VEGF Receptor 2 (phospho Tyr1214) antibody.
Lane 2 : Primary antibody pre-incubated with the antigen specific peptide

Anti-VEGF Receptor 2 (phospho Tyr1214) antibody used in IHC (Paraffin sections) (IHC-P). GTX50152

GTX50152 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50152 VEGF Receptor 2 (phospho Tyr1214) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

There are currently no references for VEGF Receptor 2 (phospho Tyr1214) antibody (GTX50152). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for VEGF Receptor 2 (phospho Tyr1214) antibody (GTX50152). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf