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VEGF antibody [VG1]

Anti-VEGF antibody [VG1] used in IHC (Paraffin sections) (IHC-P). GTX21316
Anti-VEGF antibody [VG1] used in IHC (Paraffin sections) (IHC-P). GTX21316

Cat No. GTX21316

Host Mouse
Clonality Monoclonal
Clone Name VG1
Isotype IgG1
Application WB, ICC/IF, IHC-P, IHC-Fr, ELISA
Reactivity Human, Mouse, Rat
Package
250 μl ($319)
APPLICATION

Application Note

IHC-P: 1:25 - 1:50 in ABC method. Suggested incubation period of 30 minutes at room temperature. . High temperature treatment of formalin-fixed tissue sections using 1mM EDTA, pH 8.0 must be performed prior to the immunostaining. Not yet tested in other applications. Optimal ilutions/concentrations should be determined by the end user.

Positive Control

Astrocytoma

Specificity/Sensitivity

This antibody is specific to a cluster of proteins between 19-22kD (reduced). VEGF is a homodimeric disulfide-linked glycoprotein involved in angiogenesis, which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium.
PROPERTIES

Form

Liquid

Buffer

PBS with 1% BSA and 0.05% sodium azide in it.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Antigen Species

Human

Immunogen

Recombinant VEGF protein.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

VEGFA, VPF, 125853, 7422, P15692, MGC70609, VEGF, MVCD1

Cellular Localization

Secreted

Background

VEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated.

Research Area

DATA IMAGES
Anti-VEGF antibody [VG1] used in IHC (Paraffin sections) (IHC-P). GTX21316

GTX21316 IHC-P Image

IHC Image using GTX21316 - Detection of VEGF by IHC in Human Angiosarcoma

Anti-VEGF antibody [VG1] used in IHC (Paraffin sections) (IHC-P). GTX21316

GTX21316 IHC-P Image

IHC-P analysis of human kidney using VEGF antibody [VG1].

REFERENCE
REVIEW
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Application Reference
Clone Reference
Package List Price ($)
$ 319