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VEGF antibody [VG1]

Anti-VEGF antibody [VG1] used in IHC (Paraffin sections) (IHC-P). GTX21316

Cat No. GTX21316

Host

Mouse

Clonality

Monoclonal

Clone Name

VG1

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA

Reactivity

Human, Mouse, Rat, Zebrafish
Package
250 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:20‐1:40
IHC-Fr Assay dependent
FACS Assay dependent
ELISA Assay dependent

Note :

IHC-P
Recommendations for antigen retrieval : EDTA buffer pH 8.0 at room temperature for 30 mins.

Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Product Note

This antibody is specific to a cluster of proteins between 19-22 kDa (reduced).

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

BALB/C mice were injected with a recombinant VEGF protein.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_373276

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

vascular endothelial growth factor A , MVCD1 , VEGF , VPF

Cellular Localization

Secreted

Background

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-VEGF antibody [VG1] used in IHC (Paraffin sections) (IHC-P). GTX21316

GTX21316 IHC-P Image

IHC-P analysis of human angiosarcoma tissue using GTX21316 VEGF antibody [VG1].

REFERENCE

REVIEW

VEGF antibody [VG1] Cat No. GTX21316

Rating ( Average 5 based on 6 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 4 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28811496
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28811496
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 27561355
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Angiogenesis in 2018. PMID: 29332242
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Angiogenesis in 2018. PMID: 29332242
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Angiogenesis in 2018. PMID: 29332242
Application Reference
Clone Reference
SDS
Sodium Azide.pdf
Package List Price ($)
$ 319