GeneTex
United States (US)

VEGF antibody

GTX31194_IHC-P_1_18121410_512.jpg
GTX31194_IHC-P_18121410_591.jpg

Cat No. GTX31194

Host Goat
Clonality Polyclonal
Application WB, IHC-P, ELISA, Neut
Reactivity Human
APPLICATION

Application Note

We recommend the following starting dilutions: For IHC-P: Use at a concentration of 5.0 μg/ml-15.0 μg/ml. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. For WB: Use at a concentration of 0.1 - 0.2 μg/ml. For ELISA: Use at a concentration of 0.5 - 2.0 μg/ml. For Neutralization: one-half maximal inhibition [ND50] of the biological activity of hVEGF (10 ng/ml), a concentration of 0.05 - 0.1 μg/ml of this antibody is required. Optimal dilutions should be determined experimentally by the end user.
PROPERTIES

Form

Liquid

Buffer

PBS, pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The immunogen corresponding to a region within amino acids 27 and 191 of human VEGF165.

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

VPF Antibody , VEGFA Antibody , MVCD1 Antibody , vascular endothelial growth factor A Antibody

Background

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008]
DATA IMAGES
GTX31194_IHC-P_1_18121410_512.jpg

GTX31194 IHC-P Image

IHC-P analysis of human breast invasive ductal carcinoma using VEGF antibody. An HRP-labeled polymer detection system was used with a DAB chromogen. This antibody was also tested in an HRP-biotin labeling detection system. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended.

GTX31194_IHC-P_18121410_591.jpg

GTX31194 IHC-P Image

IHC-P analysis of human breast invasive ductal carcinoma using VEGF antibody. An HRP-labeled polymer detection system was used with a DAB chromogen. This antibody was also tested in an HRP-biotin labeling detection system. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended.

Package List Price ($)
$ 319