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VEGFA antibody

Anti-VEGFA antibody used in IHC (Paraffin sections) (IHC-P). GTX74091
Anti-VEGFA antibody used in IHC (Paraffin sections) (IHC-P). GTX74091
Anti-VEGFA antibody used in IHC (Paraffin sections) (IHC-P). GTX74091
Anti-VEGFA antibody used in IHC (Paraffin sections) (IHC-P). GTX74091

Cat. No. GTX74091

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA, Functional Assay

Reactivity

 Mouse
Package
100 μg ($619)
See all VEGFA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.2μg/ml
ICC/IF 2.5μg/ml
IHC-P 2.5μg/ml
ELISA 0.5μg/ml
Functional Assay Assay dependent

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA buffer pH 8.0 is recommended for this purpose.
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Product Note

This antibody recognizes mouse Vascular Endothelial Growth Factor (VEGF). It has not been cross adsorbed and may react with homologous proteins from related species.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant mouse VEGF

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_378197

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vascular endothelial growth factor A , Vegf , Vpf

Background

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.[provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-VEGFA antibody used in IHC (Paraffin sections) (IHC-P). GTX74091

GTX74091 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX74091 VEGFA antibody.

Anti-VEGFA antibody used in IHC (Paraffin sections) (IHC-P). GTX74091

GTX74091 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX74091 VEGFA antibody.

Anti-VEGFA antibody used in IHC (Paraffin sections) (IHC-P). GTX74091

GTX74091 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX74091 VEGFA antibody.

Anti-VEGFA antibody used in IHC (Paraffin sections) (IHC-P). GTX74091

GTX74091 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX74091 VEGFA antibody.

REFERENCE

Application Reference

Chen YJ et al. Cancer Lett 2012; 319 (2) : 223-31 Progesterone receptor is involved in 2,3,7,8-tetrachlorodibenzo-p-dioxin-stimulated breast cancer cells proliferation.
Application : WB
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 619