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VEGFA antibody [4E3]

Overexpression
Anti-VEGFA antibody [4E3] used in Western Blot (WB). GTX83426
Anti-VEGFA antibody [4E3] used in IHC (Paraffin sections) (IHC-P). GTX83426
Anti-VEGFA antibody [4E3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83426
Anti-VEGFA antibody [4E3] used in IHC (Paraffin sections) (IHC-P). GTX83426
Anti-VEGFA antibody [4E3] used in Western Blot (WB). GTX83426
Anti-VEGFA antibody [4E3] used in IHC (Paraffin sections) (IHC-P). GTX83426
Anti-VEGFA antibody [4E3] used in IHC (Paraffin sections) (IHC-P). GTX83426

Cat. No. GTX83426

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4E3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($519)
See all VEGFA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 27-233 of human VEGFA (NP_003367) produced in E.coli.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_10729063

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vascular endothelial growth factor A , MVCD1 , VEGF , VPF

Cellular Localization

Secreted

Background

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

Database

Research Area

DATA IMAGES

Anti-VEGFA antibody [4E3] used in Western Blot (WB). GTX83426

GTX83426 WB Image

WB analysis of various cell lines using GTX83426 VEGFA antibody [4E3].
Loading : 10 ug per lane
Dilution : 1:200

Anti-VEGFA antibody [4E3] used in IHC (Paraffin sections) (IHC-P). GTX83426

GTX83426 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX83426 VEGFA antibody [4E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-VEGFA antibody [4E3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83426

GTX83426 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with VEGFA plasmid using GTX83426 VEGFA antibody [4E3].

Anti-VEGFA antibody [4E3] used in IHC (Paraffin sections) (IHC-P). GTX83426

GTX83426 IHC-P Image

IHC-P analysis of human breast tissue using GTX83426 VEGFA antibody [4E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-VEGFA antibody [4E3] used in Western Blot (WB). GTX83426

GTX83426 WB Image

Overexpression

WB analysis of HEK293T cells transfected with VEGFA plasmid (Right) or empty vector (Left) for 48 hrs using GTX83426 VEGFA antibody [4E3].
Loading : 5 ug per lane

Anti-VEGFA antibody [4E3] used in IHC (Paraffin sections) (IHC-P). GTX83426

GTX83426 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX83426 VEGFA antibody [4E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-VEGFA antibody [4E3] used in IHC (Paraffin sections) (IHC-P). GTX83426

GTX83426 IHC-P Image

IHC-P analysis of human liver tissue using GTX83426 VEGFA antibody [4E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

Application Reference

Quintero Sierra LA et al. Tissue Eng Regen Med 2022; Tissue-Material Integration and Biostimulation Study of Collagen Acellular Matrices.
Application : IHC-P
Reactivity : Human
Merigo F et al. Cell Tissue Res 2021; 383 (3) : 1103-1122 Immunolocalization of leptin and leptin receptor in colorectal mucosa of ulcerative colitis, Crohn's disease and control subjects with no inflammatory bowel disease.
De Francesco F et al. J Int Med Res 2020; 48 (8) : 3.00061E+14 Artificial dermal substitutes for tissue regeneration: comparison of the clinical outcomes and histological findings of two templates.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519