Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with VEGFA antibody [HL1755] (GTX637405) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.

VEGF antibody [HL1755] detects VEGF protein at vesicle by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VEGF stained by VEGF antibody [HL1755] (GTX637405) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with VEGFA antibody [HL1755] (GTX637405) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with VEGFA antibody [HL1755] (GTX637405) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 15% SDS-PAGE, and the membrane was blotted with VEGFA antibody [HL1755] (GTX637405) diluted at 1:500000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.