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VEGFC antibody [HL2289]

Recombinant antibody Orthogonal
Anti-VEGFC antibody [HL2289] used in Western Blot (WB). GTX638344
Anti-VEGFC antibody [HL2289] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638344
Anti-VEGFC antibody [HL2289] used in IHC (Paraffin sections) (IHC-P). GTX638344
Anti-VEGFC antibody [HL2289] used in IHC (Paraffin sections) (IHC-P). GTX638344

Cat. No. GTX638344

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2289

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all VEGFC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human VEGFC.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vascular endothelial growth factor C , Flt4-L , LMPH1D , LMPHM4 , VRP

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. The encoded protein promotes angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. The proprotein is further cleaved into a fully processed form that can bind and activate VEGFR-2 and VEGFR-3 receptors. [provided by RefSeq, Apr 2014]

Database

Research Area

DATA IMAGES

Anti-VEGFC antibody [HL2289] used in Western Blot (WB). GTX638344

GTX638344 WB Image

Orthogonal

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VEGFC antibody [HL2289] (GTX638344) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VEGFC antibody [HL2289] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638344

GTX638344 ICC/IF Image

VEGFC antibody [HL2289] detects VEGFC protein at cytoplasmic vesicles by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VEGFC stained by VEGFC antibody [HL2289] (GTX638344) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-VEGFC antibody [HL2289] used in IHC (Paraffin sections) (IHC-P). GTX638344

GTX638344 IHC-P Image

VEGFC antibody [HL2289] detects VEGFC protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
VEGFC stained by VEGFC antibody [HL2289] (GTX638344) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VEGFC antibody [HL2289] used in IHC (Paraffin sections) (IHC-P). GTX638344

GTX638344 IHC-P Image

VEGFC antibody [HL2289] detects VEGFC protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
VEGFC stained by VEGFC antibody [HL2289] (GTX638344) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations