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VEGFC antibody

KO/KD Orthogonal
Anti-VEGFC antibody used in Western Blot (WB). GTX113574
Anti-VEGFC antibody used in Western Blot (WB). GTX113574
Anti-VEGFC antibody used in IHC (Paraffin sections) (IHC-P). GTX113574
Anti-VEGFC antibody used in Western Blot (WB). GTX113574
Anti-VEGFC antibody used in Western Blot (WB). GTX113574

Cat No. GTX113574

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Bovine
Package
100 μl ($329),
25 μl ($169)
See all VEGFC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

TL1 , mouse lung , Rat spleen , U87-MG conditioned medium

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VEGFC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620764

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

vascular endothelial growth factor C , Flt4-L , LMPH1D , LMPHM4 , VRP

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VEGFC antibody used in Western Blot (WB). GTX113574

GTX113574 WB Image

VEGFC antibody detects VEGFC protein by western blot analysis.
A. 50 μg mouse lung lysate/extract
12% SDS-PAGE
VEGFC antibody (GTX113574) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VEGFC antibody used in Western Blot (WB). GTX113574

GTX113574 WB Image

Orthogonal

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with VEGFC antibody (GTX113574) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 15297417

Anti-VEGFC antibody used in IHC (Paraffin sections) (IHC-P). GTX113574

GTX113574 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using VEGFC(GTX113574) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VEGFC antibody used in Western Blot (WB). GTX113574

GTX113574 WB Image

KO/KD

Sample (20 μg of whole cell lysate)
A: TL1 whole cell lysate
B: TL1 knock down VEGF-C whole cell lysate
12% SDS PAGE
GTX113574 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VEGFC antibody used in Western Blot (WB). GTX113574

GTX113574 WB Image

VEGFC antibody detects VEGFC protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with VEGFC antibody (GTX113574) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wang YW et al. Transl Oncol 2021; 14 (10) : 101175 The significance of SMARCB1 in the pathogenesis of renal cell carcinoma with rhabdoid features.
Application : WB
Reactivity : Human
Zhang J et al. Ecotoxicol Environ Saf 2021; 218 : 112257 Increased secretion of VEGF-C from SiO2-induced pulmonary macrophages promotes lymphangiogenesis through the Src/eNOS pathway in silicosis.
Application : WB
Reactivity : Human
Wang CA et al. J Extracell Vesicles 2020; 9 (1) : 1746529 DUSP2 regulates extracellular vesicle-VEGF-C secretion and pancreatic cancer early dissemination.
Hayashi KG et al. Theriogenology 2019; 133 : 56-64 Temporal expression and localization of vascular endothelial growth factor family members in the bovine uterus during peri-implantation period.
Application : IHC-P
Reactivity : Bovine
Huang YL et al. Cell Physiol Biochem 2018; 50 (2) : 597-611 High Glucose Induces VEGF-C Expression via the LPA1/3-Akt-ROS-LEDGF Signaling Axis in Human Prostate Cancer PC-3 Cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169