GeneTex
United States (US)

VEGFC antibody

KO/KD Orthogonal
GTX113574_40198_IHC_18121410_486.jpg
GTX113574_40198_CT_WB_18121410_107.jpg
GTX113574_40198_20180202_WB_Fraction_18121410_263.jpg
GTX113574_40198_20150827_WB_R_spleen_18121410_577.jpg
GTX113574_40198_WB_M_Lung_18121410_237.jpg
GTX113574_43103_20180202_WB_Fraction_18121410_227.jpg

Cat No. GTX113574

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

TL1 , mouse lung , Rat spleen , U87-MG conditioned medium

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VEGFC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Flt4-L antibody, VRP antibody, VEGFC antibody, vascular endothelial growth factor-related protein antibody, VEGF-C antibody, FLT4 ligand DHM antibody, vascular endothelial growth factor C antibody

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX113574_40198_IHC_18121410_486.jpg

GTX113574 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using VEGFC(GTX113574) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113574_40198_CT_WB_18121410_107.jpg

GTX113574 WB Image

KO/KD

Sample (20 µg of whole cell lysate)
A: TL1 whole cell lysate
B: TL1 knock down VEGF-C whole cell lysate
12% SDS PAGE
GTX113574 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113574_40198_20180202_WB_Fraction_18121410_263.jpg

GTX113574 WB Image

Orthogonal

U87-MG whole cell extract and conditioned medium (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with VEGFC antibody (GTX113574) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 15297417

GTX113574_40198_20150827_WB_R_spleen_18121410_577.jpg

GTX113574 WB Image

VEGFC antibody detects VEGFC protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with VEGFC antibody (GTX113574) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113574_40198_WB_M_Lung_18121410_237.jpg

GTX113574 WB Image

VEGFC antibody detects VEGFC protein by western blot analysis.
A. 50 µg mouse lung lysate/extract
12% SDS-PAGE
VEGFC antibody (GTX113574) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113574_43103_20180202_WB_Fraction_18121410_227.jpg

GTX113574 WB Image

U87-MG whole cell extract and conditioned medium (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with VEGFC antibody (GTX113574) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 15297417

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf