GeneTex
United States (US)

VEGFC antibody

GTX113574_40198_IHC_18121410_486.jpg
GTX113574_40198_CT_WB_18121410_107.jpg
GTX113574_40198_20180202_WB_Fraction_18121410_263.jpg
GTX113574_40198_20150827_WB_R_spleen_18121410_577.jpg
GTX113574_40198_WB_M_Lung_18121410_237.jpg
GTX113574_43103_20180202_WB_Fraction_18121410_227.jpg

Cat No. GTX113574

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

TL1 , mouse lung , Rat spleen , U87-MG conditioned medium

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VEGFC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Vascular Endothelial Growth Factor C,Flt4-L,Lmph1D,Vrp,Vegfc

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX113574_40198_IHC_18121410_486.jpg

GTX113574 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using VEGFC(GTX113574) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113574_40198_CT_WB_18121410_107.jpg

GTX113574 WB Image

Sample (20 µg of whole cell lysate)
A: TL1 whole cell lysate
B: TL1 knock down VEGF-C whole cell lysate
12% SDS PAGE
GTX113574 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113574_40198_20180202_WB_Fraction_18121410_263.jpg

GTX113574 WB Image

U87-MG whole cell extract and conditioned medium (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with VEGFC antibody (GTX113574) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 15297417

GTX113574_40198_20150827_WB_R_spleen_18121410_577.jpg

GTX113574 WB Image

VEGFC antibody detects VEGFC protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with VEGFC antibody (GTX113574) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113574_40198_WB_M_Lung_18121410_237.jpg

GTX113574 WB Image

VEGFC antibody detects VEGFC protein by western blot analysis.
A. 50 µg mouse lung lysate/extract
12% SDS-PAGE
VEGFC antibody (GTX113574) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113574_43103_20180202_WB_Fraction_18121410_227.jpg

GTX113574 WB Image

U87-MG whole cell extract and conditioned medium (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with VEGFC antibody (GTX113574) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 15297417

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf