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VEGFC antibody

KO/KD Orthogonal
Anti-VEGFC antibody used in IHC (Paraffin sections) (IHC-P). GTX113574
Anti-VEGFC antibody used in IHC (Paraffin sections) (IHC-P). GTX113574
Anti-VEGFC antibody used in Western Blot (WB). GTX113574
Anti-VEGFC antibody used in IHC (Paraffin sections) (IHC-P). GTX113574
Anti-VEGFC antibody used in Western Blot (WB). GTX113574
Anti-VEGFC antibody used in Western Blot (WB). GTX113574
Anti-VEGFC antibody used in Western Blot (WB). GTX113574

Cat. No. GTX113574

Reference 7
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Bovine
Package
100 μl ($399),
25 μl ($169)
See all VEGFC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

TL1 , mouse lung , Rat spleen , U87-MG conditioned medium

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VEGFC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620764

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vascular endothelial growth factor C , Flt4-L , LMPH1D , LMPHM4 , VRP

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VEGFC antibody used in IHC (Paraffin sections) (IHC-P). GTX113574

GTX113574 IHC-P Image

VEGFC antibody detects secreted VEGFC protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
VEGFC stained by VEGFC antibody (GTX113574) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VEGFC antibody used in IHC (Paraffin sections) (IHC-P). GTX113574

GTX113574 IHC-P Image

VEGFC antibody detects secreted VEGFC protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
VEGFC stained by VEGFC antibody (GTX113574) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VEGFC antibody used in Western Blot (WB). GTX113574

GTX113574 WB Image

KO/KD

Sample (20 μg of whole cell lysate)
A: TL1 whole cell lysate
B: TL1 knock down VEGF-C whole cell lysate
12% SDS PAGE
GTX113574 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VEGFC antibody used in IHC (Paraffin sections) (IHC-P). GTX113574

GTX113574 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using VEGFC(GTX113574) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VEGFC antibody used in Western Blot (WB). GTX113574

GTX113574 WB Image

VEGFC antibody detects VEGFC protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with VEGFC antibody (GTX113574) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VEGFC antibody used in Western Blot (WB). GTX113574

GTX113574 WB Image

VEGFC antibody detects VEGFC protein by western blot analysis.
A. 50 μg mouse lung lysate/extract
12% SDS-PAGE
VEGFC antibody (GTX113574) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VEGFC antibody used in Western Blot (WB). GTX113574

GTX113574 WB Image

Orthogonal

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VEGFC antibody (GTX113574) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wang YW et al. Transl Oncol 2021; 14 (10) : 101175 The significance of SMARCB1 in the pathogenesis of renal cell carcinoma with rhabdoid features.
Application : WB
Reactivity : Human
Zhang J et al. Ecotoxicol Environ Saf 2021; 218 : 112257 Increased secretion of VEGF-C from SiO2-induced pulmonary macrophages promotes lymphangiogenesis through the Src/eNOS pathway in silicosis.
Application : WB
Reactivity : Human
Wang CA et al. J Extracell Vesicles 2020; 9 (1) : 1746529 DUSP2 regulates extracellular vesicle-VEGF-C secretion and pancreatic cancer early dissemination.
Hayashi KG et al. Theriogenology 2019; 133 : 56-64 Temporal expression and localization of vascular endothelial growth factor family members in the bovine uterus during peri-implantation period.
Application : IHC-P
Reactivity : Bovine
Huang YL et al. Cell Physiol Biochem 2018; 50 (2) : 597-611 High Glucose Induces VEGF-C Expression via the LPA1/3-Akt-ROS-LEDGF Signaling Axis in Human Prostate Cancer PC-3 Cells.
Application : WB
Reactivity : Human
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REVIEW

VEGFC antibody Cat. No. GTX113574

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-VEGFC antibody used in Western Blot (WB). GTX113574
Date : Anonymous submitted on 14-Feb-2023
Score :
Application Tested : WB
Sample Species : Rat
Sample : rat pulmonary artery smooth muscle cell lysates
Amount used : 9μg
Blocking : 5% BSA, 25°C, 1Hr
Primary Antibody : 1:500, 16°C, 4Hr
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169