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VGAT antibody [HL1616]

Recombinant antibody Orthogonal Overexpression
Anti-VGAT antibody [HL1616] used in Western Blot (WB). GTX637107
Anti-VGAT antibody [HL1616] used in IHC (Frozen sections) (IHC-Fr). GTX637107
Anti-VGAT antibody [HL1616] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637107
Anti-VGAT antibody [HL1616] used in IHC (Frozen sections) (IHC-Fr). GTX637107
Anti-VGAT antibody [HL1616] used in Western Blot (WB). GTX637107
Anti-VGAT antibody [HL1616] used in IHC (Paraffin sections) (IHC-P). GTX637107
Anti-VGAT antibody [HL1616] used in IHC (Paraffin sections) (IHC-P). GTX637107
Anti-VGAT antibody [HL1616] used in IHC (Paraffin sections) (IHC-P). GTX637107

Cat. No. GTX637107

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1616

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all VGAT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Predict Reactivity

Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VGAT. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 32 member 1 , VGAT , VIAAT

Background

The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-VGAT antibody [HL1616] used in Western Blot (WB). GTX637107

GTX637107 WB Image

Orthogonal Overexpression

Non-transfected (–) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1616] (GTX637107) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VGAT antibody [HL1616] used in IHC (Frozen sections) (IHC-Fr). GTX637107

GTX637107 IHC-Fr Image

VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100.

Anti-VGAT antibody [HL1616] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637107

GTX637107 ICC/IF Image

VGAT antibody [HL1616] detects VGAT protein at nucleus and cytoskeleton by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-VGAT antibody [HL1616] used in IHC (Frozen sections) (IHC-Fr). GTX637107

GTX637107 IHC-Fr Image

VGAT antibody [HL1616] detects VGAT protein at cell membrane by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-VGAT antibody [HL1616] used in Western Blot (WB). GTX637107

GTX637107 WB Image

Orthogonal

Boiled and unboiled mouse tissue extract (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1616] (GTX637107) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VGAT antibody [HL1616] used in IHC (Paraffin sections) (IHC-P). GTX637107

GTX637107 IHC-P Image

VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VGAT antibody [HL1616] used in IHC (Paraffin sections) (IHC-P). GTX637107

GTX637107 IHC-P Image

VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100.
Red: NF-H, a Cytoplasm marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VGAT antibody [HL1616] used in IHC (Paraffin sections) (IHC-P). GTX637107

GTX637107 IHC-P Image

VGAT antibody [HL1616] detects VGAT protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations