GeneTex
United States (US)

VGAT antibody [N1N2], N-term

GTX101908_40275_20161004_IFA_19011506_460.jpg
GTX101908_40275_20170214_IHC-Fr_19011506_329.jpg
GTX101908_42837_20170522_IHC-P_M_19011506_567.jpg
GTX101908_40275_IHC_19011506_166.jpg
GTX101908_40275_20170531_IHC-Fr_M_19011506_548.jpg
GTX101908_42837_20170713_WB_B_19011506_945.jpg

Cat No. GTX101908

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.

Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

SLC32A1-transfected 293T

Predict Reactivity

Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human VGAT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Solute Carrier Family 32 Member 1,Vgat,Viaat,Slc32A1

Cellular Localization

Cytoplasmic vesicle membrane; Multi-pass membrane protein

Background

The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101908_40275_20161004_IFA_19011506_460.jpg

GTX101908 ICC/IF Image

VGAT antibody [N1N2], N-term detects VGAT protein expression by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VGAT protein stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101908_40275_20170214_IHC-Fr_19011506_329.jpg

GTX101908 IHC-Fr Image

VGAT antibody [N1N2], N-term detects VGAT protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: VGAT protein stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101908_42837_20170522_IHC-P_M_19011506_567.jpg

GTX101908 IHC-P Image

VGAT antibody [N1N2], N-term detects VGAT protein expression by immunohistochemical analysis.
Sample:Paraffin-Embedded adult mouse retina.
Green: VGAT protein stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101908_40275_IHC_19011506_166.jpg

GTX101908 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using VGAT(GTX101908) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101908_40275_20170531_IHC-Fr_M_19011506_548.jpg

GTX101908 IHC-Fr Image

VGAT antibody [N1N2], N-term detects VGAT protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: VGAT protein stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101908_42837_20170713_WB_B_19011506_945.jpg

GTX101908 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169