VGAT antibody [N1N2], N-term

VGAT antibody [N1N2], N-term detects VGAT protein expression by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VGAT protein stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

VGAT antibody [N1N2], N-term detects VGAT protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: VGAT protein stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Boiled and unboiled mouse tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using VGAT(GTX101908) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

VGAT antibody [N1N2], N-term detects VGAT protein by immunofluorescent analysis. Sample: DIV9 rat cortical neuron and Glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGAT stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

VGAT antibody [N1N2], N-term detects VGAT protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: VGAT protein stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

VGAT antibody [N1N2], N-term detects VGAT protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: VGAT stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min