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VGAT antibody [N1N2], N-term

Orthogonal Overexpression
Anti-VGAT antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101908
Anti-VGAT antibody [N1N2], N-term used in IHC (Frozen sections) (IHC-Fr). GTX101908
Anti-VGAT antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101908
Anti-VGAT antibody [N1N2], N-term used in IHC (Frozen sections) (IHC-Fr). GTX101908
Anti-VGAT antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101908
Anti-VGAT antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101908
Anti-VGAT antibody [N1N2], N-term used in Western Blot (WB). GTX101908
Anti-VGAT antibody [N1N2], N-term used in Western Blot (WB). GTX101908
Anti-VGAT antibody [N1N2], N-term used in Western Blot (WB). GTX101908

Cat. No. GTX101908

Reference 2
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419),
25 μl ($169)
See all VGAT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Predict Reactivity

Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human VGAT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619521

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 32 member 1 , VGAT , VIAAT

Cellular Localization

Cytoplasmic vesicle membrane; Multi-pass membrane protein

Background

The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VGAT antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101908

GTX101908 ICC/IF Image

VGAT antibody [N1N2], N-term detects VGAT protein expression by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VGAT protein stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-VGAT antibody [N1N2], N-term used in IHC (Frozen sections) (IHC-Fr). GTX101908

GTX101908 IHC-Fr Image

VGAT antibody [N1N2], N-term detects VGAT protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: VGAT protein stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-VGAT antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101908

GTX101908 IHC-P Image

VGAT antibody [N1N2], N-term detects VGAT protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: VGAT stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VGAT antibody [N1N2], N-term used in IHC (Frozen sections) (IHC-Fr). GTX101908

GTX101908 IHC-Fr Image

VGAT antibody [N1N2], N-term detects VGAT protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: VGAT protein stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-VGAT antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101908

GTX101908 ICC/IF Image

VGAT antibody [N1N2], N-term detects VGAT protein by immunofluorescent analysis. Sample: DIV9 rat cortical neuron and Glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGAT stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250. Red: Tau, a Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

Anti-VGAT antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101908

GTX101908 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using VGAT(GTX101908) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VGAT antibody [N1N2], N-term used in Western Blot (WB). GTX101908

GTX101908 WB Image

Overexpression

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VGAT antibody [N1N2], N-term used in Western Blot (WB). GTX101908

GTX101908 WB Image

Orthogonal

Boiled and unboiled mouse tissue extract (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VGAT antibody [N1N2], N-term used in Western Blot (WB). GTX101908

GTX101908 WB Image

Orthogonal

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Schmeichel AM et al. Sleep 2021; Loss of putative GABAergic neurons in the ventrolateral medulla in multiple system atrophy.
Sato M et al. Neuropathol Appl Neurobiol 2020; (Epub) Ataxic phenotype and neurodegeneration are triggered by the impairment of chaperone-mediated autophagy in cerebellar neurons.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

VGAT antibody [N1N2], N-term Cat. No. GTX101908

Rating
( Average 5 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Anti-VGAT antibody [N1N2], N-term used in IHC (Frozen sections) (IHC-Fr). GTX101908
Date : Anonymous submitted on 27-Apr-2022
Score :
Application Tested : IHC-Fr
Sample Species : Rat
Sample : Rat brain tissue section
Blocking : 5% BSA in 1xPB, 25°C, 0.5Hr
Fixation : 10% PFA in 1xPB
Permeabilization /Antigen retrieval : Immunosaver (Nisshin-EM), 40min, 95°C
Primary Antibody : 1:1000, 25°C, 12Hr
Figure Note : GABAA receptor beta3 subunit (Green, clone[N87/25]) and VGAT (Red, GTX101908) in the rat medulla neuropile.
The signal for the GABA receptor was detected on a neuron’s cell surface, while the signal for VGAT was detected over the GABA receptors, likely in the pre-synapses onto the neuon.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 419
$ 169