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VGF antibody

Anti-VGF antibody used in IHC (Paraffin sections) (IHC-P). GTX85398
Anti-VGF antibody used in IHC (Paraffin sections) (IHC-P). GTX85398
Anti-VGF antibody used in Western Blot (WB). GTX85398

Cat. No. GTX85398

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all VGF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Human Brain Tissue Lysate(GTX27918)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

VGF antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human VGF.The immunogen is located within the last 50 amino acids of VGF.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

VGF nerve growth factor inducible , SCG7 , SgVII

Cellular Localization

Secreted

Background

VGF was initially identified as a rapidly regulated gene product in nerve growth factor-treated PC12 cells. This protein is synthesized in neurons in the central and peripheral nervous system as well as in the pituitary, adrenal medulla, endocrine cells of the stomach, and pancreatic beta cells. VGF is thought to be involved in organism energy balance and regulation of homeostasis as mice lacking this gene show deficits in these areas. More recent results suggest that VGF is upregulated by brain-derived neurotrophic factor (BDNF) and can stimulate the proliferation of hippocampal progenitor cells and produce antidepressant-like behavioral effects, suggesting that this pathway may be a suitable target for therapeutic treatments.

Database

Research Area

DATA IMAGES

Anti-VGF antibody used in IHC (Paraffin sections) (IHC-P). GTX85398

GTX85398 IHC-P Image

IHC-P analysis of human brain tissue using GTX85398 VGF antibody.
Working concentration : 20 μg/ml

Anti-VGF antibody used in IHC (Paraffin sections) (IHC-P). GTX85398

GTX85398 IHC-P Image

IHC-P analysis of human brain tissue using GTX85398 VGF antibody.
Working concentration : 5 μg/ml

Anti-VGF antibody used in Western Blot (WB). GTX85398

GTX85398 WB Image

WB analysis of human brain tissue lysate using GTX85398 VGF antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399