GeneTex

  • Country / Location Selection

United States (US)

VGLUT2 antibody

Anti-VGLUT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133142
Anti-VGLUT2 antibody used in Western Blot (WB). GTX133142
Anti-VGLUT2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133142

Cat. No. GTX133142

Reference 1
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, IHC (Free Floating)

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all VGLUT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC (Free Floating) Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse VGLUT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886841

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6 , 2900073D12Rik , DNPI , VGLUT2

Cellular Localization

Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane , Membrane; Multi-pass membrane protein (Potential) , Cell junction , synapse , synaptosome

Background

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.

Database

Research Area

DATA IMAGES

Anti-VGLUT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133142

GTX133142 ICC/IF Image

VGLUT2 antibody detects VGLUT2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VGLUT2 stained by VGLUT2 antibody (GTX133142) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-VGLUT2 antibody used in Western Blot (WB). GTX133142

GTX133142 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VGLUT2 antibody (GTX133142) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VGLUT2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133142

GTX133142 IHC-Fr Image

VGLUT2 antibody detects VGLUT2 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: VGLUT2 stained by VGLUT2 antibody (GTX133142) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

REFERENCE

Application Reference

L Li et al. Exp Neurol 2023; 372 : 114616 Neonatal di-(2-ethylhexyl)phthalate exposure induces permanent alterations in secretory CRH neuron characteristics in the hypothalamus paraventricular region of adult male rats
Application : WB
Submit a Reference

REVIEW

VGLUT2 antibody Cat. No. GTX133142

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
IHC (Free Floating)(IHC (Free Floating))
( Average 5 based on 1 users reviews)
Anti-VGLUT2 antibody used in Western Blot (WB). GTX133142
Date : Anonymous submitted on 14-Feb-2023
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse brain tissue lysate
Amount used : 10μg
Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:2000, 16°C, 25Hr
Anti-VGLUT2 antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX133142
Date : Anonymous submitted on 25-May-2021
Score :
Application Tested : IHC (Free Floating)
Sample Species : Ms
Sample : Mouse Brain cerebellum 250μm slice
Fixation : 4% PFA in PBS, 4°C, 16Hr
Permeabilization /Antigen retrieval : 2% Triton X-100 in PBS, 25°C, 48Hr
Primary Antibody : 1:100, 4°C, 48Hr
Figure Note : VGLUT2 (green) & Calbindin (red, GTX634833)
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169