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VGLUT2 antibody

Anti-VGLUT2 antibody used in Western Blot (WB). GTX54871
Anti-VGLUT2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54871
Anti-VGLUT2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54871

Cat. No. GTX54871

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all VGLUT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide (C)EDGKPLEVPEKK, corresponding to amino acid residues 45-56 (Cytoplasmic, N-terminus) of rat VGLUT2 (Accession  : Q9JI12).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 17 member 6 , Dnpi , Vglut2

Cellular Localization

Membrane

Background

Na+-dependent inorganic phosphate cotransporter; involved in Ca2+-dependent glutamate release from astrocytes [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-VGLUT2 antibody used in Western Blot (WB). GTX54871

GTX54871 WB Image

WB analysis of rat (lanes 1 and 4), mouse brain membrane (lanes 2 and 5), and SH-SY5Y cell (lanes 3 and 6) lysates using GTX54871 VGLUT2 antibody preincubated with or without immunogen peptide.
Dilution : 1:400

Anti-VGLUT2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54871

GTX54871 IHC-Fr Image

IHC-Fr analysis of rat spinal cord tissue using GTX54871 VGLUT2 antibody. Cell nuclei were stained with DAPI (blue).
Panel A : Vesicular glutamate transporter 2 labeling (green).
Panel B : The same section labeled for P2X7 receptor (orange).
Panel C : Merge of A and B demonstrates partial co-localization of VGLUT2 and P2X7 receptor in dorsal horn and in lateral column (L. Col., arrow).
Dilution : 1:100

Anti-VGLUT2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54871

GTX54871 IHC-Fr Image

IHC-Fr analysis of rat neocortex tissue using GTX54871 VGLUT2 antibody. VGLUT2 (red) is detected in pyramidal neurons (arrow). The same section was stained with DAPI (blue).
Dilution : 1:400

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639