Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of mouse VGluT1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2801548
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 , 2900052E22Rik , AI851913 , Vglut1
Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
VGluT1 antibody detects VGluT1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133148 IHC-P Image
VGluT1 antibody detects VGluT1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133148 ICC/IF Image
VGluT1 antibody detects VGluT1 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX133148 IHC-P Image
VGluT1 antibody detects VGluT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Show More +
GTX133148 IHC-P Image
VGluT1 antibody detects VGluT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Show More +
GTX133148 ICC/IF Image
VGluT1 antibody detects VGluT1 protein by immunofluorescent analysis.Sample: DIV9 rat cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:250.Red: Tau, a Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
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GTX133148 IHC-P Image
VGluT1 antibody detects VGluT1 protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. VGluT1 antibody (GTX133148) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133148 IHC-Fr Image
VGluT1 antibody detects VGluT1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: VGluT1 protein stained by VGluT1 antibody (GTX133148) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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GTX133148 IHC-P Image
VGluT1 antibody detects VGluT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Show More +
GTX133148 IHC-P Image
VGluT1 antibody detects VGluT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Show More +
GTX133148 IHC-P Image
VGluT1 antibody detects VGluT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Show More +
GTX133148 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VGluT1 antibody (GTX133148) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133148 WB Image
Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VGluT1 antibody (GTX133148) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.