VGluT1 antibody

Name: VGluT1 antibody
Brand: GeneTex
SKU: GTX133148
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-VGluT1 antibody used in Western Blot (WB). GTX133148

Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148

Anti-VGluT1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133148

Anti-VGluT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133148

Cat. No. GTX133148

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all VGluT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000

Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse VGluT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2801548

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 , 2900052E22Rik , AI851913 , Vglut1

Cellular Localization

Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane , Membrane; Multi-pass membrane protein (Potential) , Cell junction , synapse , synaptosome

Background

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.

Database

Gene ID: 72961 Slc17a7
UniProt: Q3TXX4 Slc17a7

Research Area

DATA IMAGES

GTX133148 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VGluT1 antibody (GTX133148) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133148 IHC-Fr Image

VGluT1 antibody detects VGluT1 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: VGluT1 protein stained by VGluT1 antibody (GTX133148) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133148 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VGluT1 antibody (GTX133148) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133148 ICC/IF Image

VGluT1 antibody detects VGluT1 protein by immunofluorescent analysis.
Sample: DIV9 rat cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:250.
Red: Tau, a Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
VGluT1 antibody (GTX133148) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133148 ICC/IF Image

VGluT1 antibody detects VGluT1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Liu Z et al. Biomater Res 2023; 27 (1) : 108 Nanoarchitectonics of tannic acid based injectable hydrogel regulate the microglial phenotype to enhance neuroplasticity for poststroke rehabilitation.

Seiya Yamada et al. iScience 2020; 23 (5) : 101058 Nwd1 Regulates Neuronal Differentiation and Migration through Purinosome Formation in the Developing Cerebral Cortex.

Application : IHC-Fr

Reactivity : Mouse

Submit a Reference

REVIEW

VGluT1 antibody Cat. No. GTX133148

Rating

( Average 5 based on 1 users reviews)

Application

Western Blot(WB)

( Average 5 based on 1 users reviews)

Date : Anonymous submitted on 14-Feb-2023

Score :

Application Tested : WB

Sample Species : Ms

Sample : Mouse brain tissue lysate

Amount used : 2.5μg

Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr

Primary Antibody : 1:2000, 16°C, 25Hr

Submit a Review

Application Reference

Liu Z et al. Biomater Res 2023; 27 (1):108 Nanoarchitectonics of tannic acid based injectable hydrogel regulate the microglial phenotype to enhance neuroplasticity for poststroke rehabilitation.

Seiya Yamada et al. iScience 2020; 23 (5):101058 Nwd1 Regulates Neuronal Differentiation and Migration through Purinosome Formation in the Developing Cerebral Cortex.

Application : IHC-Fr

Reactivity : Mouse

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

Package	List Price ($)
100 μl	$ 399
25 μl	$ 169
Qty

VGluT1 antibody

Cat. No. GTX133148

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX133148 WB Image

GTX133148 IHC-P Image

GTX133148 IHC-Fr Image

GTX133148 IHC-P Image

GTX133148 IHC-P Image

GTX133148 WB Image

GTX133148 IHC-P Image

GTX133148 IHC-P Image

GTX133148 IHC-P Image

GTX133148 ICC/IF Image

GTX133148 IHC-P Image

GTX133148 IHC-P Image

GTX133148 ICC/IF Image

REFERENCE

Application Reference

REVIEW

VGluT1 antibody Cat. No. GTX133148

Product

Research

Support

Company

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