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VGluT1 antibody

Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148
Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148
Anti-VGluT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133148
Anti-VGluT1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133148
Anti-VGluT1 antibody used in Western Blot (WB). GTX133148

Cat No. GTX133148

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all VGluT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse VGluT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 , 2900052E22Rik , AI851913 , Vglut1

Cellular Localization

Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane , Membrane; Multi-pass membrane protein (Potential) , Cell junction , synapse , synaptosome

Background

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.

Database

Research Area

DATA IMAGES

Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
VGluT1 antibody (GTX133148) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VGluT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133148

GTX133148 ICC/IF Image

VGluT1 antibody detects VGluT1 protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VGluT1 protein stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-VGluT1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133148

GTX133148 IHC-Fr Image

VGluT1 antibody detects VGluT1 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: VGluT1 protein stained by VGluT1 antibody (GTX133148) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-VGluT1 antibody used in Western Blot (WB). GTX133148

GTX133148 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VGluT1 antibody (GTX133148) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Seiya Yamada et al. iScience 2020; 23 (5) : 101058 Nwd1 Regulates Neuronal Differentiation and Migration through Purinosome Formation in the Developing Cerebral Cortex.
Application : IHC-Fr
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169