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VGluT1 antibody

Orthogonal
Anti-VGluT1 antibody used in Western Blot (WB). GTX133148
Anti-VGluT1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133148
Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148
Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148
Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148
Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148
Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148
Anti-VGluT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133148
Anti-VGluT1 antibody used in Western Blot (WB). GTX133148
Anti-VGluT1 antibody used in Western Blot (WB). GTX133148
Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148
Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148

Cat. No. GTX133148

Reference 2
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all VGluT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of mouse VGluT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2801548

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 , 2900052E22Rik , AI851913 , Vglut1

Cellular Localization

Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane , Membrane; Multi-pass membrane protein (Potential) , Cell junction , synapse , synaptosome

Background

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.

Database

Research Area

DATA IMAGES

Anti-VGluT1 antibody used in Western Blot (WB). GTX133148

GTX133148 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VGluT1 antibody (GTX133148) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VGluT1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133148

GTX133148 IHC-Fr Image

VGluT1 antibody detects VGluT1 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: VGluT1 protein stained by VGluT1 antibody (GTX133148) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
VGluT1 antibody (GTX133148) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VGluT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133148

GTX133148 ICC/IF Image

VGluT1 antibody detects VGluT1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-VGluT1 antibody used in Western Blot (WB). GTX133148

GTX133148 WB Image

Orthogonal

Boiled and unboiled various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VGluT1 antibody (GTX133148) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VGluT1 antibody used in Western Blot (WB). GTX133148

GTX133148 WB Image

Orthogonal

Boiled and unboiled rat tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VGluT1 antibody (GTX133148) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Liu Z et al. Biomater Res 2023; 27 (1) : 108 Nanoarchitectonics of tannic acid based injectable hydrogel regulate the microglial phenotype to enhance neuroplasticity for poststroke rehabilitation.
Seiya Yamada et al. iScience 2020; 23 (5) : 101058 Nwd1 Regulates Neuronal Differentiation and Migration through Purinosome Formation in the Developing Cerebral Cortex.
Application : IHC-Fr
Reactivity : Mouse
Submit a Reference

REVIEW

VGluT1 antibody Cat. No. GTX133148

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-VGluT1 antibody used in Western Blot (WB). GTX133148
Date : Anonymous submitted on 14-Feb-2023
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse brain tissue lysate
Amount used : 2.5μg
Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:2000, 16°C, 25Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169