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VIP antibody

Anti-VIP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129461
Anti-VIP antibody used in IHC (Frozen sections) (IHC-Fr). GTX129461
Anti-VIP antibody used in Western Blot (WB). GTX129461
Anti-VIP antibody used in IHC (Paraffin sections) (IHC-P). GTX129461
Anti-VIP antibody used in Western Blot (WB). GTX129461

Cat No. GTX129461

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

SK-N-SH , SK-N-AS , PC-3

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VIP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Vasoactive Intestinal Peptide,Phm27,Vip

Cellular Localization

Secreted

Background

The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-VIP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129461

GTX129461 ICC/IF Image

VIP antibody detects VIP protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VIP stained by VIP antibody (GTX129461) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-VIP antibody used in IHC (Frozen sections) (IHC-Fr). GTX129461

GTX129461 IHC-Fr Image

VIP antibody detects VIP protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse thalamus.
Green: VIP stained by VIP antibody (GTX129461) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-VIP antibody used in Western Blot (WB). GTX129461

GTX129461 WB Image

VIP antibody detects VIP protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with VIP antibody (GTX129461) at a dilution of 1:1000.

Anti-VIP antibody used in IHC (Paraffin sections) (IHC-P). GTX129461

GTX129461 IHC-P Image

VIP antibody detects VIP protein at cytosol on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
VIP antibody (GTX129461) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VIP antibody used in Western Blot (WB). GTX129461

GTX129461 WB Image

VIP antibody detects VIP protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with VIP antibody (GTX129461) diluted at 1:1000.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169