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VIP antibody

Anti-VIP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129461
Anti-VIP antibody used in IHC (Paraffin sections) (IHC-P). GTX129461
Anti-VIP antibody used in IHC (Paraffin sections) (IHC-P). GTX129461
Anti-VIP antibody used in Western Blot (WB). GTX129461
Anti-VIP antibody used in Western Blot (WB). GTX129461
Anti-VIP antibody used in Western Blot (WB). GTX129461
Anti-VIP antibody used in IHC (Frozen sections) (IHC-Fr). GTX129461

Cat. No. GTX129461

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all VIP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

PC-3

Predict Reactivity

Zebrafish, Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VIP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886003

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vasoactive intestinal peptide , PHM27

Cellular Localization

Secreted

Background

The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VIP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129461

GTX129461 ICC/IF Image

VIP antibody detects VIP protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VIP stained by VIP antibody (GTX129461) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-VIP antibody used in IHC (Paraffin sections) (IHC-P). GTX129461

GTX129461 IHC-P Image

VIP antibody detects VIP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
VIP stained by VIP antibody (GTX129461) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VIP antibody used in IHC (Paraffin sections) (IHC-P). GTX129461

GTX129461 IHC-P Image

VIP antibody detects VIP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
VIP stained by VIP antibody (GTX129461) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VIP antibody used in Western Blot (WB). GTX129461

GTX129461 WB Image

Rat tissue extracts (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with VIP antibody (GTX129461) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VIP antibody used in Western Blot (WB). GTX129461

GTX129461 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with VIP antibody (GTX129461) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VIP antibody used in Western Blot (WB). GTX129461

GTX129461 WB Image

VIP antibody detects VIP protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with VIP antibody (GTX129461) at a dilution of 1:1000.

Anti-VIP antibody used in IHC (Frozen sections) (IHC-Fr). GTX129461

GTX129461 IHC-Fr Image

VIP antibody detects VIP protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse thalamus.
Green: VIP stained by VIP antibody (GTX129461) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Wu CY et al. Biology (Basel) 2023; 12 (10) Klotho Null Mutation Indirectly Leads to Age-Related Lacrimal Gland Degeneration in Mutant Mice.
Broome ST et al. J Mol Neurosci 2023; 73 (9月10日) : 724-737 Early Alterations of PACAP and VIP Expression in the Female Rat Brain Following Spinal Cord Injury.
De-Fong Huang et al. 2022; 119 (33) Neuronal splicing regulator RBFOX3 mediates seizures via regulating Vamp1 expression preferentially in NPY-expressing GABAergic neurons.
Application : IHC-Fr
Reactivity : Mouse
Musumeci G et al. Int J Mol Sci 2018; 19 (4) Identification of Dysregulated microRNA Networks in Schwann Cell-Like Cultures Exposed to Immune Challenge: Potential Crosstalk with the Protective VIP/PACAP Neuropeptide System.
Application : WB
Reactivity : Rat
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169