GeneTex
United States (US)

VIP antibody

GTX129461_41570_20181004_ICC_IF_R_19011519_802.jpg
GTX129461_41570_20150320_WB_18121410_429.jpg
GTX129461_41570_IHC_M_18121410_301.jpg
GTX129461_41570_20151029_WB_18121410_427.jpg

Cat No. GTX129461

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

SK-N-SH , SK-N-AS , PC-3

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VIP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Vasoactive Intestinal Peptide,Phm27,Vip

Cellular Localization

Secreted

Background

The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX129461_41570_20181004_ICC_IF_R_19011519_802.jpg

GTX129461 ICC/IF Image

VIP antibody detects VIP protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VIP stained by VIP antibody (GTX129461) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX129461_41570_20150320_WB_18121410_429.jpg

GTX129461 WB Image

VIP antibody detects VIP protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with VIP antibody (GTX129461) at a dilution of 1:1000.

GTX129461_41570_IHC_M_18121410_301.jpg

GTX129461 IHC-P Image

VIP antibody detects VIP protein at cytosol on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
VIP antibody (GTX129461) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX129461_41570_20151029_WB_18121410_427.jpg

GTX129461 WB Image

VIP antibody detects VIP protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with VIP antibody (GTX129461) diluted at 1:1000.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169