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VISA antibody

Anti-VISA antibody used in IHC (Paraffin sections) (IHC-P). GTX85480
Anti-VISA antibody used in Western Blot (WB). GTX85480
Anti-VISA antibody used in IHC (Paraffin sections) (IHC-P). GTX85480

Cat. No. GTX85480

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all VISA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

Rat Brain Tissue Lysate(GTX27942)

Product Note

This antibody is specific for VISA Internal

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

VISA antibody was raised against a 17 amino acid synthetic peptide from near the center of human VISA.The immunogen is located within amino acids 130 - 180 of VISA.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitochondrial antiviral signaling protein , CARDIF , IPS-1 , IPS1 , VISA

Cellular Localization

Mitochondrion outer membrane,Mitochondrion

Background

Two distinct signaling pathways activate the host innate immunity against viral infection. One pathway is reliant on members of the Toll-like receptor (TLR) family while the other uses the RNA helicase RIG-I as a receptor for intracellular viral double-stranded RNA as a trigger for the immune response. VISA is a mitochondrial membrane protein that was identified as a critical component in the IFN-b signaling pathways that recruits IRF-3 to RIG-I, leading to its activation and that of NF-kappaB. VISA is also thought to interact with other components of the innate immune pathway such as the TLR adapter protein TRIF, TRAF2 and TRAF6. VISA also interacts with the IKKalpha, IKKbeta and IKKepsilon kinases through its C-terminal region. Cleavage of this region by the Hepatitis C virus (HCV) protease allows HCV to escape the host immune system. At least three isoforms of VISA are known to exist.

Database

Research Area

DATA IMAGES

Anti-VISA antibody used in IHC (Paraffin sections) (IHC-P). GTX85480

GTX85480 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX85480 VISA antibody.
Working concentration : 2.5 μg/ml

Anti-VISA antibody used in Western Blot (WB). GTX85480

GTX85480 WB Image

WB analysis of rat brain tissue lysate using GTX85480 VISA antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

Anti-VISA antibody used in IHC (Paraffin sections) (IHC-P). GTX85480

GTX85480 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX85480 VISA antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399