APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 3-6μg/ml |
|
Note :
IHC-P
Human Kidney shows brushborder staning in PCT cells and Human Pancreas shows cytoplasm staining of beta cells in islets of Langerhans.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89450-PEP).
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-HALQEKDTQTKLE, from the internal region of the protein sequence according to NP_068375.3.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
VPS11 core subunit of CORVET and HOPS complexes , END1 , HLD12 , PEP5 , RNF108 , hVPS11
Cellular Localization
Endosome , Lysosome membrane , Cytoplasmic vesicle
Background
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Database
Research Area
DATA IMAGES
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GTX89450 IHC-P Image
IHC-P analysis of human kidney- using GTX89450 VPS11 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml
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GTX89450 IHC-P Image
IHC-P analysis of human pancreas using GTX89450 VPS11 antibody, Internal.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 4μg/ml
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REFERENCE
There are currently no references for VPS11 antibody, Internal (GTX89450). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for VPS11 antibody, Internal (GTX89450). Be the first to share your experience with this product.
