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VPS11 antibody

Anti-VPS11 antibody used in IHC (Paraffin sections) (IHC-P). GTX107706
Anti-VPS11 antibody used in Western Blot (WB). GTX107706
Anti-VPS11 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107706

Cat. No. GTX107706

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all VPS11 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

293T , HepG2

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human VPS11. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241443

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

VPS11 core subunit of CORVET and HOPS complexes , END1 , HLD12 , PEP5 , RNF108 , hVPS11

Cellular Localization

Late endosome membrane; Peripheral membrane protein; Cytoplasmic side , Lysosome membrane; Peripheral membrane protein; Cytoplasmic side

Background

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VPS11 antibody used in IHC (Paraffin sections) (IHC-P). GTX107706

GTX107706 IHC-P Image

VPS11 antibody detects VPS11 protein at cytosol on Ca922 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Ca922 xenograft.
VPS11 antibody (GTX107706) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VPS11 antibody used in Western Blot (WB). GTX107706

GTX107706 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:Hep G2(GTX27900)
7.5% SDS PAGE
GTX107706 diluted at 1:500

Anti-VPS11 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107706

GTX107706 ICC/IF Image

VPS11 antibody detects VPS11 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20ºC 100% MeOH for 5 min.
Green: VPS11 protein stained by VPS11 antibody (GTX107706) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169