GeneTex
United States (US)

VPS11 antibody

GTX107706_39715_IFA_19011507_538.jpg
GTX107706_WB_19011507_670.jpg
GTX107706_39715_IHC_19011507_476.jpg

Cat No. GTX107706

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

293T , HepG2

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human VPS11. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Vps11, Corvet/Hops Core Subunit,End1,Hld12,Pep5,Rnf108,Hvps11,Vps11

Cellular Localization

Late endosome membrane; Peripheral membrane protein; Cytoplasmic side , Lysosome membrane; Peripheral membrane protein; Cytoplasmic side

Background

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107706_39715_IFA_19011507_538.jpg

GTX107706 ICC/IF Image

VPS11 antibody detects VPS11 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20℃ 100% MeOH for 5 min.
Green: VPS11 protein stained by VPS11 antibody (GTX107706) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX107706_WB_19011507_670.jpg

GTX107706 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:Hep G2(GTX27900)
7.5% SDS PAGE
GTX107706 diluted at 1:500

GTX107706_39715_IHC_19011507_476.jpg

GTX107706 IHC-P Image

VPS11 antibody detects VPS11 protein at cytosol on Ca922 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Ca922 xenograft.
VPS11 antibody (GTX107706) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169